大鼠肝脏亚细胞组分对还原型谷胱甘肽的氧化作用。
Oxidation of reduced glutathione by subcellular fractions of rat liver.
作者信息
Christophersen B O
出版信息
Biochem J. 1966 Jul;100(1):95-101. doi: 10.1042/bj1000095.
Abstract
- A new method was used to diminish the autoxidation of GSH. 2. The oxidation of GSH by liver homogenates was studied with regard to concentration of homogenate, concentration of GSH, time, pH and anaerobiosis. 3. GSH was oxidized by recombinations of the supernatant with microsomes and with mitochondria. Each fraction alone caused little oxidation. 4. Proteins in the supernatant were required to obtain the effect, and low-molecular-weight compounds in the same fraction increased its effect. 5. GSH diminished the formation of malonaldehyde in homogenates. 6. GSH prevented a stimulating effect of the supernatant on the formation of malonaldehyde in microsomes and in mitochondria. 7. The malonaldehyde formation in microsomes together with the supernatant did not start until the concentration of endogenous low-molecular-weight thiols had decreased to a low level. 8. It is suggested that part of the oxidation of GSH in homogenates is coupled to a mechanism that counteracts the peroxidation of membrane lipids.
摘要
- 采用了一种新方法来减少谷胱甘肽（GSH）的自氧化。2. 就匀浆浓度、GSH浓度、时间、pH值和厌氧状态等方面研究了肝匀浆对GSH的氧化作用。3. 上清液与微粒体及线粒体的混合物可氧化GSH。单独每个组分引起的氧化作用很小。4. 需要上清液中的蛋白质才能产生这种效应，同一组分中的低分子量化合物可增强其效应。5. GSH可减少匀浆中丙二醛的形成。6. GSH可阻止上清液对微粒体和线粒体中丙二醛形成的刺激作用。7. 微粒体与上清液一起时，直到内源性低分子量硫醇浓度降至低水平才开始形成丙二醛。8. 有人提出，匀浆中GSH的部分氧化与一种对抗膜脂过氧化的机制相关联。
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