大鼠肝脏匀浆中亚细胞组分在谷胱甘肽氧化中的作用。
The function of subcellular fractions in the oxidation of glutathione in rat liver homogenate.
作者信息
Jocelyn P C
出版信息
Biochem J. 1970 May;117(5):951-6. doi: 10.1042/bj1170951.
Abstract
- The aerobic loss of GSH added to the supernatant fraction from rat liver is much increased by including the microsome fraction, which both inhibits the concurrent reduction of the GSSG formed and also augments the net oxidation rate. 2. Oxidation occurs with a mixture of dialysed supernatant and a protein-free filtrate; the latter is replaceable by hypoxanthine and the former by xanthine oxidase, whereas fractions lacking this enzyme give no oxidation. 3. In all these instances augmentation occurs with microsomes, with fractions having urate oxidase activity and with the purified enzyme; uric acid and microsomes alone also support the oxidation. 4. Evidence implicating additional protein factors is discussed. 5. It is suggested that GSH oxidation by homogenate is linked through glutathione peroxidase to the reaction of endogenous substrate with supernatant xanthine oxidase and of the uric acid formed with peroxisomal urate oxidase.
摘要
- 向大鼠肝脏上清液组分中添加的谷胱甘肽（GSH）的需氧损失，因加入微粒体组分而大大增加，微粒体组分既抑制了同时形成的氧化型谷胱甘肽（GSSG）的还原，又提高了净氧化速率。2. 透析后的上清液与无蛋白滤液的混合物会发生氧化反应；后者可用次黄嘌呤替代，前者可用黄嘌呤氧化酶替代，而缺乏该酶的组分则不会发生氧化。3. 在所有这些情况下，微粒体、具有尿酸氧化酶活性的组分以及纯化的酶都会促进氧化反应；单独的尿酸和微粒体也能支持氧化反应。4. 讨论了涉及其他蛋白质因子的证据。5. 有人提出，匀浆对谷胱甘肽的氧化作用是通过谷胱甘肽过氧化物酶，与内源性底物与上清液黄嘌呤氧化酶的反应以及所形成的尿酸与过氧化物酶体尿酸氧化酶的反应相联系的。
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本文引用的文献
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The intracellular localization of uricase.尿酸酶的细胞内定位。
J Biol Chem. 1951 May;190(1):331-7.
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Intracellular distribution of xanthine oxidase in the rat liver.大鼠肝脏中黄嘌呤氧化酶的细胞内分布
Nature. 1955 Jun 18;175(4468):1087. doi: 10.1038/1751087a0.
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Comparison of the aerobic and anaerobic reduction of oxidized glutathione in liver homogenates.肝脏匀浆中氧化型谷胱甘肽的需氧和厌氧还原比较。
Biochem J. 1961 Apr;79(1):43-51. doi: 10.1042/bj0790043.
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Detection of free radicals generated during enzymic oxidations by the initiation of sulfite oxidation.通过亚硫酸盐氧化引发来检测酶促氧化过程中产生的自由基。
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Enzymatic activity of cytoplasmic particulates of rat liver isolated by gradient centrifugation.通过梯度离心分离的大鼠肝脏细胞质颗粒的酶活性。
Arch Biochem Biophys. 1954 Aug;51(2):487-98. doi: 10.1016/0003-9861(54)90504-4.
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Hydrogen peroxide detoxication by glutathione peroxidase and catalase in rat liver homogenates.大鼠肝脏匀浆中谷胱甘肽过氧化物酶和过氧化氢酶对过氧化氢的解毒作用。
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