Wuthier R E
J Lipid Res. 1966 Jul;7(4):544-50.
Two-dimensional chromatography on commercially available silica gel-loaded paper for the microanalysis of polar lipids from various tissues is described. All common phospholipids and their lyso derivatives can be reproducibly separated. As many as 22 lipid components were separated on a single chromatogram. Improved methods for staining lipids and for determining phosphorus in the chromatographic spots are reported.
描述了使用市售硅胶负载纸进行二维色谱分析以对各种组织中的极性脂质进行微量分析的方法。所有常见的磷脂及其溶血衍生物都能被重复分离。在一张色谱图上可分离出多达22种脂质成分。还报告了改进的脂质染色方法和色谱斑点中磷的测定方法。
