Department of Agronomy, University of Illinois, Urbana, Illinois 61801.
Plant Physiol. 1977 Apr;59(4):587-90. doi: 10.1104/pp.59.4.587.
Mesophyll cells were rapidly isolated from soybean (Glycine max [L.]) leaves using a combined Macerase enzyme-stirring technique. About 50% to 70% of the leaf cells on a chlorophyll basis from 3 grams of leaves could be isolated in 15 minutes. The cells obtained by this method were capable of high rates of photosynthesis even after storage in the dark for periods of up to 9 hours. The CO(2)-saturated rate of photosynthesis increased from 5 mum CO(2)/mg Chl.hour at 5 C to 170 mum CO(2)/mg Chl.hour at 40 C. At atmospheric CO(2) concentration, the rate varied from 5 to 55 mum CO(2)/mg Chl.hour over this temperature range. The reduced temperature response of photosynthesis at low CO(2) concentration was due to an increased Km(CO(2)) of the cells with increasing temperature. The products of photosynthesis in the isolated cells were similar to the products of leaf photosynthesis.
采用纤维素酶搅拌法从大豆（Glycine max [L.])叶片中快速分离出叶肉细胞。3 克叶片可在 15 分钟内分离出约 50%至 70%的基于叶绿素的叶片细胞。用这种方法获得的细胞即使在黑暗中储存长达 9 小时，光合作用的速率也很高。CO2 饱和光合作用速率从 5°C 时的 5 mum CO2/mg Chl.hour 增加到 40°C 时的 170 mum CO2/mg Chl.hour。在大气 CO2 浓度下，该速率在该温度范围内从 5 到 55 mum CO2/mg Chl.hour 变化。在低 CO2 浓度下，光合作用对低温的响应是由于细胞的 Km(CO2)随温度升高而增加。分离细胞中光合作用的产物与叶片光合作用的产物相似。
