Johnston S D, Hayden D W, Kiang D T, Handschin B, Johnson K H
Am J Vet Res. 1984 Feb;45(2):379-82.
Estradiol and progesterone receptors were measured in tumor cytosols from 3 intact and 4 neutered female cats with spontaneously occurring mammary adenocarcinomas. Serum from 2 of the intact cats which had been in estrus 4 and 4 to 6 weeks before tumor excision contained progesterone concentrations of 16.2 and 2.2 ng/ml, respectively; serum progesterone in the other cats was less than 2 ng/ml. Estradiol receptors were not detected in any cytosols. Progesterone receptors were detected in all of the cytosols, in concentrations ranging from 4.0 to 11.7 (mean = 7.2) fmol/mg of protein. Scatchard plot analysis of tumor cytosol from an 8th cat with mammary adenocarcinoma revealed presence of high affinity progesterone binding with a dissociation constant (Kd) of 3.47 nM. Tumor receptor content could not be correlated with stage of the estrous cycle nor with whether the cat was intact or neutered.
对3只未绝育和4只已绝育的患有自发性乳腺腺癌的雌性猫的肿瘤细胞溶质进行了雌二醇和孕酮受体检测。2只未绝育猫在肿瘤切除前4周以及4至6周处于发情期，其血清孕酮浓度分别为16.2和2.2 ng/ml；其他猫的血清孕酮浓度低于2 ng/ml。在所有细胞溶质中均未检测到雌二醇受体。在所有细胞溶质中均检测到孕酮受体，浓度范围为4.0至11.7（平均 = 7.2）fmol/mg蛋白质。对第8只患有乳腺腺癌的猫的肿瘤细胞溶质进行Scatchard图分析，结果显示存在高亲和力孕酮结合，解离常数（Kd）为3.47 nM。肿瘤受体含量与发情周期阶段以及猫是否未绝育或已绝育均无相关性。
