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[牛布鲁氏菌病的变态反应诊断。2. 变态反应试验在感染畜群中的应用(作者译)]

[Allergic diagnosis of bovine brucellosis. 2. Use of the allergic test in infected herds (author's transl)].

作者信息

Fensterbank R

出版信息

Ann Rech Vet. 1977;8(2):195-201.

PMID:596798
Abstract

Four hundred and eighty-one cows, in 27 herds, were injected intradermally with a brucella purified protein allergen in order to study the value of the skin test in the diagnosis of bovine brucellosis. The serology was done on the serum or plasma from 444 of these cows. Two hundred and nine cows showed an allergic reaction and 174 were positive in serology. One hundred and twenty-three were simultaneously positive to both tests, 52 were positive in serology but did not show any allergic reaction and 66 showed an allergic reaction but were negative in serology. In general, brucellosis was certainly detected at the herd level with this test. In newly-infected herds, the skin test gives a more complete and earlier result than serological tests. The use of the skin test is recommended as a supplemental test when unknown animals are to be introduced in non-infected herds, to improve the detection of contaminated animals in newly-infected herds and to solve the case of problem herds.

摘要

为了研究皮肤试验在牛布鲁氏菌病诊断中的价值,对27个牛群中的481头奶牛进行了布鲁氏菌纯化蛋白变应原皮内注射。对其中444头奶牛的血清或血浆进行了血清学检测。209头奶牛出现过敏反应,174头血清学检测呈阳性。123头奶牛两项检测均呈阳性,52头血清学检测呈阳性但未出现任何过敏反应,66头出现过敏反应但血清学检测呈阴性。总体而言,通过该检测在畜群水平上肯定能检测出布鲁氏菌病。在新感染的畜群中,皮肤试验比血清学检测能给出更完整、更早的结果。当要将未知动物引入未感染畜群时,建议使用皮肤试验作为补充检测,以提高新感染畜群中受污染动物的检测率,并解决问题畜群的情况。

相似文献

1
[Allergic diagnosis of bovine brucellosis. 2. Use of the allergic test in infected herds (author's transl)].[牛布鲁氏菌病的变态反应诊断。2. 变态反应试验在感染畜群中的应用(作者译)]
Ann Rech Vet. 1977;8(2):195-201.
2
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Comparison of enzyme-labeled immunosorbent assay and particle concentration fluorescence immunoassay with standard serologic methods and bacteriologic culture for detection of Brucella sp-infected cows in herds with brucellosis.酶联免疫吸附测定和颗粒浓度荧光免疫测定与标准血清学方法及细菌学培养在布鲁氏菌病疫区检测布鲁氏菌感染奶牛中的比较
J Am Vet Med Assoc. 1993 Jun 15;202(12):1975-7.

引用本文的文献

1
Characterization of a monoclonal antibody specific for Brucella smooth lipopolysaccharide and development of a competitive enzyme-linked immunosorbent assay to improve the serological diagnosis of brucellosis.一种针对布鲁氏菌光滑型脂多糖的单克隆抗体的特性鉴定以及一种竞争性酶联免疫吸附测定法的开发,以改进布鲁氏菌病的血清学诊断。
Clin Diagn Lab Immunol. 1996 May;3(3):309-14. doi: 10.1128/cdli.3.3.309-314.1996.
2
Brucella ribosomal protein L7/L12 is a major component in the antigenicity of brucellin INRA for delayed-type hypersensitivity in brucella-sensitized guinea pigs.布鲁氏菌核糖体蛋白L7/L12是布鲁氏菌素INRA抗原性的主要成分,该抗原可引起布鲁氏菌致敏豚鼠的迟发型超敏反应。
Infect Immun. 1994 Dec;62(12):5361-6. doi: 10.1128/iai.62.12.5361-5366.1994.
3
Specific bovine brucellosis diagnosis based on in vitro antigen-specific gamma interferon production.基于体外抗原特异性γ干扰素产生的牛布鲁氏菌病特异性诊断。
J Clin Microbiol. 1995 Mar;33(3):706-12. doi: 10.1128/jcm.33.3.706-712.1995.
4
Comparison of the brucellin skin test with the lymphocyte transformation test in bovine brucellosis.布鲁菌素皮肤试验与淋巴细胞转化试验在牛布鲁氏菌病中的比较。
J Hyg (Lond). 1986 Jun;96(3):403-13. doi: 10.1017/s002217240006616x.