Fensterbank R, Pardon P
Ann Rech Vet. 1977;8(2):187-93.
A protein allergen extracted from the rough strain Brucella melitensis B 115, prepared for the diagnosis of Brucellosis, has been evaluated in cows. Injected in non infected and non vaccinated cows, this allergen does not give any reaction, does not sensitize for subsequent injections, does not give rise to antibodies detected by routine serologic tests, and thus does not interfere with usual screening. In cows, sensitized by injections of H 38 or 45/20 vaccines, the intradermal injection produces a delayed hypersensitivity reaction. The recommanded dose was determined to be 100 microgram and the reaction must be read at 72 hours. The most suitable sites of injection are the neck or the tail fold. It appears likely that allergic testing could not be employed for diagnosis in cows vaccinated with H 38 or 45/20 vaccines. Allergic testing is useful in cows which have been given strain 19 vaccine at least two years previously.
从粗糙型布鲁氏菌羊种B 115中提取的一种用于布鲁氏菌病诊断的蛋白质过敏原已在奶牛身上进行了评估。将这种过敏原注射到未感染且未接种疫苗的奶牛体内，不会引起任何反应，不会使奶牛对后续注射产生致敏，也不会产生常规血清学检测可检测到的抗体，因此不会干扰常规筛查。在通过注射H 38或45/20疫苗致敏的奶牛中，皮内注射会产生迟发型超敏反应。推荐剂量确定为100微克，反应必须在72小时时读取。最合适的注射部位是颈部或尾褶。对于接种了H 38或45/20疫苗的奶牛，似乎无法采用过敏试验进行诊断。过敏试验对至少在两年前接种过19号菌株疫苗的奶牛有用。
