从海拉细胞中分离中期染色体。
Isolation of metaphase chromosomes from HeLa cells.
作者信息
Huberman J A, Attardi G
出版信息
J Cell Biol. 1966 Oct;31(1):95-105. doi: 10.1083/jcb.31.1.95.
Abstract
The authors have developed a method for large-scale isolation of metaphase chromosomes from HeLa cells. The distinguishing feature of this method is the use of a pH sufficiently low (about 3) to stabilize the chromosomes against mechanical damage. Many milligrams of fairly pure, morphologically intact chromosomes can be isolated in 8 hr or less of total working time. The isolated chromosomes contain about 2.0 mg of acid-soluble protein, 2.7 mg of acid-insoluble protein and 0.66 mg of RNA for each milligram of DNA. The RNA bound to the isolated chromosomes consists mainly of ribosomal RNA, but there is also a significant amount of 45S RNA.
摘要
作者们开发了一种从HeLa细胞中大规模分离中期染色体的方法。该方法的显著特点是使用足够低的pH值（约为3）来稳定染色体，防止其受到机械损伤。在总共8小时或更短的工作时间内，可以分离出许多毫克相当纯净、形态完整的染色体。每毫克DNA，分离出的染色体含有约2.0毫克酸溶性蛋白质、2.7毫克酸不溶性蛋白质和0.66毫克RNA。与分离出的染色体结合的RNA主要由核糖体RNA组成，但也有大量的45S RNA。
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