Bayliss C E, Crawford F B, Nsafoah B
Can J Physiol Pharmacol. 1977 Dec;55(6):1358-68. doi: 10.1139/y77-181.
Classical models for assessing myocardial function suffer from either a potentially anoxic central core or an unsophisticated perfusion system. We have improved Langer's perfused interventricular septum model by adapting it for parabiotic resuscitation, thus achieving rapid stability and significantly less edema. The new model, which consists of a triangular piece of rabbit interventricular septum, perfused through its septal artery, first undergoes a stabilization period of 30 min during parabiotic perfusion from another rabbit. An arteriovenous shunt is then opened and the preparation remains stable for 2 h of perfusion in which fresh blood is recirculated nonparabiotically. It is proposed that during this period, experimental interventions could be undertaken using each septum as its own control. The behaviour of 66 preparations was consistent with classical concepts of myocardial function. Simple measurements were identified for beat-to-beat functional assessment. Contractility was measured by maximum active tension and maximum differential of tension. Relaxation was measured by maximum negative differential of tension and, in the presence of ischemic contracture, changes in resting tension. Compliance was measured by septal length at a specified resting tension. Perfusability was measured by the relationship between pressure and flow.
用于评估心肌功能的传统模型存在潜在的缺氧核心或不完善的灌注系统问题。我们通过将兰格氏灌注室间隔模型应用于联体复苏进行了改进，从而实现了快速稳定且水肿明显减轻。新模型由一块三角形的兔室间隔组成，通过其间隔动脉进行灌注，首先在来自另一只兔子的联体灌注期间经历30分钟的稳定期。然后打开动静脉分流，制剂在新鲜血液非联体循环灌注2小时的过程中保持稳定。有人提出，在此期间，可以将每个室间隔作为自身对照进行实验干预。66个制剂的行为与心肌功能的经典概念一致。确定了用于逐搏功能评估的简单测量方法。收缩性通过最大主动张力和最大张力差来测量。舒张通过最大负张力差来测量，在存在缺血性挛缩的情况下，通过静息张力的变化来测量。顺应性通过特定静息张力下的间隔长度来测量。灌注能力通过压力与流量之间的关系来测量。
