Wang Y S, Byrd C S, Barlaz M A
Department of Civil Engineering, North Carolina State University, Raleigh 27695-7908.
J Ind Microbiol. 1994 May;13(3):147-53. doi: 10.1007/BF01583999.
Improved techniques are needed to predict potential methane generation from refuse buried in landfills. The Biochemical Methane Potential (BMP) test was used to measure the methane potential of ten refuse samples excavated from a Berkeley, CA, landfill. The test was conducted in 125-ml serum bottles containing phosphate-buffered medium and inoculated with anaerobically digested sewage sludge. Comparison of the measured BMP to the theoretical BMP calculated from measured cellulose and hemicellulose concentrations indicated that cellulose plus hemicellulose is not well correlated with the measured BMP. The average of the measured to theoretical BMP was 19.1% (range 0-53%, s.d. = 16.9%). Measured sulfate concentrations showed that sulfate was an insignificant electron sink in the samples tested. Once methane production from the refuse was complete, 0.072 g of Whatman no. 1 filter paper was added to two of the four serum bottles incubated for each sample. An average of 84.9% (s.d. = 2.5%) of the added filter paper was recovered as methane, suggesting that some cellulose and hemicellulose present in refuse is recalcitrant or otherwise not bioavailable.
需要改进技术来预测填埋垃圾中潜在的甲烷生成量。采用生化甲烷潜力（BMP）测试来测量从加利福尼亚州伯克利市一个垃圾填埋场挖掘出的10个垃圾样品的甲烷潜力。该测试在装有磷酸盐缓冲培养基并接种了厌氧消化污水污泥的125毫升血清瓶中进行。将测得的BMP与根据测得的纤维素和半纤维素浓度计算出的理论BMP进行比较，结果表明纤维素加半纤维素与测得的BMP相关性不佳。测得的BMP与理论BMP的平均值为19.1%（范围为0 - 53%，标准差 = 16.9%）。测得的硫酸盐浓度表明，在测试的样品中硫酸盐是一个微不足道的电子受体。每个样品在四个血清瓶中培养，当垃圾的甲烷生成完成后，向其中两个血清瓶中加入0.072克Whatman 1号滤纸。平均有84.9%（标准差 = 2.5%）的添加滤纸转化为甲烷，这表明垃圾中存在的一些纤维素和半纤维素是难降解的或以其他方式不可生物利用的。
