Witschi H, Cöté M G
Chem Biol Interact. 1977 Dec;19(3):279-89. doi: 10.1016/0009-2797(77)90051-5.
Mice were injected i.p. with 250 or 400 mg/kg of butylated hydroxytoluene (BHT). In vivo incorporation of thymidine into pulmonary DNA was measured on days 1-7 after BHT. 2, 3 and 4 days after BHT, DNA synthesis was inhibited by a 24-h exposure to 100% oxygen, whereas on days 5, 6 and 7 after BHT, oxygen failed to depress synthesis. A similar pattern was observed when incorporation of leucine into protein was measured: 2 and 4 days after BHT, oxygen decreased leucine incorporation, but had no effect 6 days after BHT or in animals not pretreated with BHT. It is concluded that the cells proliferating early after BHT, the type II alveolar cells, are more susceptible to the cytotoxic effects of oxygen than are interstitial and capillary endothelial cells.
给小鼠腹腔注射250或400毫克/千克的丁基羟基甲苯（BHT）。在BHT处理后的第1至7天，测量胸苷在肺DNA中的体内掺入情况。BHT处理后2、3和4天，暴露于100%氧气24小时会抑制DNA合成，而在BHT处理后的第5、6和7天，氧气未能抑制合成。在测量亮氨酸掺入蛋白质的情况时也观察到了类似的模式：BHT处理后2和4天，氧气会降低亮氨酸掺入，但在BHT处理后6天或未用BHT预处理的动物中没有影响。得出的结论是，BHT处理后早期增殖的细胞，即II型肺泡细胞，比间质细胞和毛细血管内皮细胞更容易受到氧气的细胞毒性作用。
