Witschi H, Kacew S, Tsang B K, Williamson D
Chem Biol Interact. 1976 Jan;12(1):29-40. doi: 10.1016/0009-2797(76)90064-8.
Male mice of 7 different strains were injected i.p. with 400 mg/kg of butylated hydroxytoluene (BHT). 2 and 4 days later, the incorporation of thymidine into pulmonary DNA was significantly increased in all treated animals and this was accompanied by an increase in lung weight and pulmonary DNA. Thymidine kinase activity and DNA polymerase activity were enhanced in the lungs of BHT-treated animals and maximum activity of these enzymes appeared to precede maximum thymidine incorporation by 24 h. 3 days after BHT a good correlation was found between administered dose and thymidine kinase activity. Measuring the activity of this enzyme might serve as a convenient biochemical marker to follow and to quantitate BHT-produced cell proliferation in lung. The concentrations of cyclic AMP and the activity of adenylate cyclase were not altered by BHT on days 1-9 after administration. BHT produced also some dose-dependent, time-dependent increases in the activities of pulmonary 5'-nucleotidase and glucose-6-phosphate dehydrogenase (G6PDH), but had little effect on isocitric dehydrogenase (ICDH), pyruvate kinase (PK) and lactic dehydrogenase (LDH).
给7种不同品系的雄性小鼠腹腔注射400毫克/千克的丁基羟基甲苯（BHT）。2天和4天后，所有接受处理的动物肺DNA中胸苷掺入量显著增加，同时肺重量和肺DNA也增加。BHT处理动物的肺中胸苷激酶活性和DNA聚合酶活性增强，这些酶的最大活性似乎比胸苷掺入量的最大值提前24小时出现。BHT处理3天后，给药剂量与胸苷激酶活性之间存在良好的相关性。测量这种酶的活性可能是一种方便的生化标记，用于追踪和定量BHT在肺中产生的细胞增殖。给药后1 - 9天，BHT对环磷酸腺苷浓度和腺苷酸环化酶活性没有影响。BHT还使肺5'-核苷酸酶和葡萄糖-6-磷酸脱氢酶（G6PDH）的活性产生了一些剂量和时间依赖性的增加，但对异柠檬酸脱氢酶（ICDH）、丙酮酸激酶（PK）和乳酸脱氢酶（LDH）影响很小。
