Anikó K, Lajos K
Morphol Igazsagugyi Orv Sz. 1976 Jul;16(3):211-7.
Basic proteins of the granules of mast cells in nativ, formalin-, alcohol- and aceton fixed preparations without any preliminary treatment, when stained with acidic dye at the pH 9 cytochemically seem to be masked. After various preliminary treatment (treatment with acid, with cetylpiridinumchlorid, CPC) mast-cell granula stained with acidic-dye at pH 9 appear intensively acidophile. This phenomenon can be explained by the presence of basic proteins in the mast cell granula. Preliminary treatment with CPC inhibits acid radicals of the heparin. This may lead to the disintegration of the linkage between proteins of the heparin, thus amino-group of the basic proteins become reactivated and can be identified by acidic dyes. It can not be excluded as well, that CPC linked to the heparin with free positive radicals reveals acidic-dye-binding capacity. In cases of preliminary treatment with various acids this mechanism does not seem possible to lay on the base of changing of the dye binding capacity.
在未经任何预处理的天然、经福尔马林、酒精和丙酮固定的制剂中，肥大细胞颗粒的碱性蛋白，当在pH 9条件下用酸性染料进行细胞化学染色时，似乎被掩盖了。经过各种预处理（用酸、十六烷基氯化吡啶鎓，即CPC处理）后，在pH 9条件下用酸性染料染色的肥大细胞颗粒呈现出强烈的嗜酸性。这种现象可以用肥大细胞颗粒中存在碱性蛋白来解释。用CPC进行预处理会抑制肝素的酸性基团。这可能导致肝素蛋白质之间的连接解体，从而碱性蛋白的氨基重新被激活，并能被酸性染料识别。同样也不能排除，带有游离正电荷基团与肝素结合的CPC具有酸性染料结合能力。在用各种酸进行预处理的情况下，这种机制似乎不太可能基于染料结合能力的改变。
