Kelényi G, Kiss A
Acta Morphol Acad Sci Hung. 1976;24(3):237-45.
Cetylpyridinium chloride (CPC) and cetyltrimethylammonium bromide (CETAB) are bound to polyanionic substances by ionic bonds between the positively charged nitrogen of the quaternary salts and the negative groups of polyanions. The mast cell granules and some other structures treated with CPC or CETAB react selectively with acid dyes and fluorochromes. In ultrathin sections treated with CPC, phosphotungstic acid (PTA) greatly enhances the electron density of the granules of mast cells. The possible mechanism of acid dye and PTA binding by CPC or CETAB treated tissues is discussed.
十六烷基氯化吡啶（CPC）和十六烷基三甲基溴化铵（CETAB）通过季铵盐带正电荷的氮与聚阴离子的负基团之间的离子键与聚阴离子物质结合。用CPC或CETAB处理过的肥大细胞颗粒和其他一些结构会与酸性染料和荧光染料发生选择性反应。在用CPC处理的超薄切片中，磷钨酸（PTA）大大提高了肥大细胞颗粒的电子密度。本文讨论了经CPC或CETAB处理的组织与酸性染料和PTA结合的可能机制。
