Burg K, Collins A R, Johnson R T
J Cell Sci. 1977 Dec;28:29-48. doi: 10.1242/jcs.28.1.29.
We have examined the effects of hydroxyurea on u.v.-irradiated Chinese hamster CHO-KI cells. Ultraviolet irradiation followed by incubation with hydroxyurea causes only slight disruption of DNA and chromosome structure in CHO-KI cells compared with HeLa cells. There is, however, a clear potentiation by hydroxyurea of the u.v. killing of CHO-KI cells, which is most pronounced at those points in the cycle which are reported to have small DNA precursor pools. This potentiation is reduced when DNA precursors are provided together with hydroxyurea. These data are discussed in terms of an uncoupling of excision and repair DNA synthesis.
我们研究了羟基脲对紫外线照射的中国仓鼠CHO-K1细胞的影响。与HeLa细胞相比，紫外线照射后再用羟基脲孵育，CHO-K1细胞中DNA和染色体结构仅受到轻微破坏。然而，羟基脲对紫外线杀死CHO-K1细胞有明显的增强作用，在细胞周期中据报道DNA前体池较小的那些点最为明显。当DNA前体与羟基脲一起提供时，这种增强作用会减弱。根据切除和修复DNA合成的解偶联来讨论这些数据。
