Filatov M V, Sheĭkina T A
Tsitologiia. 1984 Apr;26(4):450-7.
A technique has been elaborated for analyzing the kinetics of excision repair of DNA. Previously it has been shown that inhibitors of DNA synthesis (1-beta-arabinofuranosylcytosine and hydroxyurea), taken together, drastically sensibilize human cells to the killing effects of DNA damaging agents. This sensibilization was found to depend on the ability of the cells to excision repair. If the time between the action of damaging factors and the incubation of cells in the mixture of inhibitors is extended, the rate of cell death is seen diminishing, because part of the primary damages is repaired. The dependence of residual damages on the time interval between the moment of damaging and the treatment by inhibitors reflects the kinetics of repair of DNA damages in cells. This method can be used for studying preparation at very low doses of damaging agent and even of spontaneous damages.
已精心设计出一种用于分析DNA切除修复动力学的技术。此前已表明，DNA合成抑制剂（1-β-D-阿拉伯呋喃糖基胞嘧啶和羟基脲）联合使用时，会使人类细胞对DNA损伤剂的杀伤作用极度敏感。发现这种敏感性取决于细胞的切除修复能力。如果延长损伤因素作用与细胞在抑制剂混合物中孵育之间的时间，细胞死亡率会降低，因为部分初始损伤得到了修复。残留损伤对损伤时刻与抑制剂处理之间时间间隔的依赖性反映了细胞中DNA损伤的修复动力学。该方法可用于研究极低剂量损伤剂甚至自发损伤的情况。
