Knauf S, Urbach G I
Am J Obstet Gynecol. 1977 Apr 1;127(7):705-10. doi: 10.1016/0002-9378(77)90242-3.
Human ovarian tumor-associated antigen (TAA) has been purified from ovarian tumor tissue by affinity chromatography on concanavallin A-Sepharose and three different gamma globulin-Sepharose columns. The resulting ovarian TAA appears to be contaminated by one normal antigen or family of antigens. Rabbit antiserum prepared against this purified ovarian TAA (antiserum 404) was coupled to CNBr-activated Sepharose 4B. This coupled Sepharose was added to fractionated serum from ovarian cancer patients with Stage III and IV malignancy. Bound protein was eluted with 0.2M glycine buffer and tested against antiserum 404. The bound protein contained TAA identical to the TAA isolated from ovarian tumor tissue.
人卵巢肿瘤相关抗原（TAA）已通过在伴刀豆球蛋白A-琼脂糖和三种不同的γ球蛋白-琼脂糖柱上进行亲和层析，从卵巢肿瘤组织中纯化出来。所得的卵巢TAA似乎被一种正常抗原或一组抗原污染。用这种纯化的卵巢TAA制备的兔抗血清（抗血清404）与溴化氰活化的琼脂糖4B偶联。将这种偶联的琼脂糖加入到患有III期和IV期恶性肿瘤的卵巢癌患者的分级血清中。用0.2M甘氨酸缓冲液洗脱结合的蛋白质，并针对抗血清404进行检测。结合的蛋白质中所含的TAA与从卵巢肿瘤组织中分离出的TAA相同。
