Niekus H G, de Vries W, Stouthamer A H
J Gen Microbiol. 1977 Dec;103(2):215-22. doi: 10.1099/00221287-103-2-215.
Campylobacter sputorum subspecies bubulus was grown in batch cultures in which the dissolved oxygen tension (d.o.t) was maintained at various constant levels. At a range of d.o.t. from 0.002 to 0.05 atm, which allowed good growth (mean generation time approximately 1.5 h), L-lactate was preferentially consumed before D-lactate. L-lactate oxidation was accompanied by equimolar acetate production during exponential growth. A value for YL-lactate (g dry weight bacteria per mol L-lactate) of 54 was determined. Net acetate production stopped when C. sputorum started to use D-lactate after consumption of L-lactate. When a culture growing exponentially at the expense of L-lactate was shifted from a d.o.t. of 0.02 atm to a d.o.t. of 0.15 atm, growth was impaired, and L-lactate consumption and corresponding acetate production diminished. This decrease correlated with a loss of lactate dehydrogenase activity after the shift. Campylobacter sputorum appeared to possess cytochromes of the b- and c-type and a carbon monoxide-binding pigment. Evidence is given that the principal site of oxygen damage is lactate dehydrogenase rather than the cytochrome chain.
泡林弯曲菌泡亚种在分批培养中生长，其中溶解氧张力（d.o.t）维持在不同的恒定水平。在0.002至0.05个大气压的一系列d.o.t下，细胞生长良好（平均世代时间约为1.5小时），在此期间L-乳酸优先于D-乳酸被消耗。在指数生长期间，L-乳酸氧化伴随着等摩尔乙酸盐的产生。测定的YL-乳酸值（每摩尔L-乳酸产生的细菌干重克数）为54。当泡林弯曲菌在消耗完L-乳酸后开始利用D-乳酸时，乙酸盐的净产生停止。当以L-乳酸为底物进行指数生长的培养物从0.02个大气压的d.o.t转移到0.15个大气压的d.o.t时，生长受到损害，L-乳酸的消耗和相应的乙酸盐产生减少。这种减少与转移后乳酸脱氢酶活性的丧失相关。泡林弯曲菌似乎拥有b型和c型细胞色素以及一种一氧化碳结合色素。有证据表明，氧损伤的主要部位是乳酸脱氢酶而不是细胞色素链。
