用于胎儿弯曲杆菌生长和气耐受性的改良培养基。
Improved media for growth and aerotolerance of Campylobacter fetus.
作者信息
George H A, Hoffman P S, Smibert R M, Krieg N R
出版信息
J Clin Microbiol. 1978 Jul;8(1):36-41. doi: 10.1128/jcm.8.1.36-41.1978.
The microaerophilic nature of Campylobacter fetus has complicated its recovery from human and animal sources. In this study, modifications of brucella agar and broth were tested for enhancement of growth and aerotolerance of 64 strains of C. fetus, representing each subspecies. Brucella agar supplemented with 0.025% each FeSO4 7H2O, sodium metabisulfite, and sodium pyruvate, supported growth of 98, 77, and 63% of the strains at 6% O2, 17% O2, and 21% O2, respectively. Unsupplemented brucella agar supported growth of 94, 48, and 20% of the strains. Brucella broth supplemented with 0.2% FeSO4.7H2O, 0.025% sodium metabisulfite, and 0.05% sodium pyruvate supported growth of 98% of the strains at 21% O2, compared to 75% with unsupplemented brucella broth. With both the supplemented agar and broth, growth responses occurred 1 to 2 days earlier than usual. Growth and aerotolerance of three strains of Campylobacter sputorum subsp. bubulus were not enhanced by the supplements.
胎儿弯曲杆菌的微需氧特性使其从人和动物源中分离培养变得复杂。在本研究中，对布鲁氏菌琼脂和肉汤进行了改良，以测试其对64株代表各亚种的胎儿弯曲杆菌生长和耐氧性的增强作用。添加了0.025%的七水硫酸亚铁、焦亚硫酸钠和丙酮酸钠的布鲁氏菌琼脂，分别在6%氧气、17%氧气和21%氧气条件下，支持了98%、77%和63%的菌株生长。未添加的布鲁氏菌琼脂分别支持了94%、48%和20%的菌株生长。添加了0.2%七水硫酸亚铁、0.025%焦亚硫酸钠和0.05%丙酮酸钠的布鲁氏菌肉汤在21%氧气条件下支持了98%的菌株生长，而未添加的布鲁氏菌肉汤仅支持75%的菌株生长。使用添加后的琼脂和肉汤时，生长反应比平时提前1至2天出现。补充剂并未增强三株唾液弯曲杆菌亚种牛型弯曲杆菌的生长和耐氧性。
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