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氧气对梅毒螺旋体呼吸及葡萄糖分解代谢的影响。

Influence of oxygen on respiration and glucose catabolism by Treponema pallidum.

作者信息

Barbieri J T, Cox C D

出版信息

Infect Immun. 1981 Mar;31(3):992-7. doi: 10.1128/iai.31.3.992-997.1981.

Abstract

Oxygen consumption by Treponema pallidum was observed to be glucose dependent. Treponemes consumed O2 to a low dissolved O2 concentration of 0.01 mumol of O2 per ml when respiration ceased. A fermentative degradation of glucose occurred during the maintenance of treponemes at 0.01 mumol of O2 per ml. Although the initial rate of respiration was independent of the dissolved O2 concentration, the respiration rate decreased upon prolonged incubation at 0.20 mumol of O2 per ml as compared with the respiration rate at 0.7 mumol of O2 per ml. Oxygen was determined to be the major electron acceptor during the oxidative metabolism of glucose. Acetate formation paralleled the respiration rate of the treponemes, whereas lactate production appeared to be independent of respiration. Accumulation of pyruvate occurred at 0.20 mumol of O2 per ml but not at 0.07 mumol/ml. Analyses of enzyme activities in cell-free extracts from treponemes maintained at either 0.07 or 0.20 mumol of O2 per ml for 4 h revealed a loss of pyruvate decarboxylase activity at the higher dissolved O2 concentration, suggesting the inactivation of pyruvate oxidase.

摘要

观察到梅毒螺旋体的耗氧量依赖于葡萄糖。当呼吸停止时,螺旋体将氧气消耗至低溶解氧浓度,即每毫升0.01微摩尔氧气。在将螺旋体维持在每毫升0.01微摩尔氧气的条件下,葡萄糖发生发酵性降解。尽管初始呼吸速率与溶解氧浓度无关,但与每毫升0.7微摩尔氧气时的呼吸速率相比,在每毫升0.20微摩尔氧气的条件下长时间孵育后,呼吸速率下降。已确定氧气是葡萄糖氧化代谢过程中的主要电子受体。醋酸盐的形成与螺旋体的呼吸速率平行,而乳酸的产生似乎与呼吸无关。丙酮酸在每毫升0.20微摩尔氧气时积累,但在每毫升0.07微摩尔氧气时不积累。对在每毫升0.07或0.20微摩尔氧气的条件下维持4小时的螺旋体细胞游离提取物中的酶活性进行分析,结果显示在较高的溶解氧浓度下丙酮酸脱羧酶活性丧失,这表明丙酮酸氧化酶失活。

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