Shcherbakova E Ia, Rezvaia M N
Mikrobiologiia. 1977 Nov-Dec;46(6):1064-9.
Diploid organisms appeared spontaneously in heterokaryons of Aspergillus niger produced by crossing a beige mutant, which was adenine-deficient, and a green mutant requiring the addition of yeast autolysate. The frequency of diploidization increased two-fold under the action of colchicine (0.5%). The diploids became prototrophous, the colour of their conidia increased 1.5--2.4 times. The rate of production of citric acid by the diploid strains which had originated under the action of colchinine was 1.5 times higher than that of the parent haploid mutants.
通过将一种缺乏腺嘌呤的米色突变体与一种需要添加酵母自溶物的绿色突变体杂交产生的黑曲霉异核体中自发出现了二倍体生物。在秋水仙碱（0.5%）的作用下，二倍体化频率增加了两倍。二倍体变成了原养型，其分生孢子的颜色增加了1.5至2.4倍。在秋水仙碱作用下产生的二倍体菌株的柠檬酸产量比亲本单倍体突变体高1.5倍。
