Vincent W F, Cameron J A
J Bacteriol. 1967 Jan;93(1):156-8. doi: 10.1128/jb.93.1.156-158.1967.
The amount of thiobarbiturate-reacting material in 51 strains of bacteria and three yeasts was determined. Reactive material was found to be present in all of the gram-negative bacteria examined. It was assumed that the reactive material in this case was primarily 2-keto-3-deoxyoctonate (KDO), an eight-carbon sugar acid which is usually associated with the cell wall lipolysaccharide of members of the Salmonella-Escherichia group. Very little reactive material could be detected in the gram-positive species and yeasts that were examined. When expressed as per cent dry weight, the gram-negative bacteria exhibited about eight times more reactive material than the gram-positive species. It is suggested that the small amount of reactive material detected in gram-positive cells and yeasts is due to compounds other than KDO.
测定了51株细菌和3株酵母中硫代巴比妥酸反应性物质的含量。在所检测的所有革兰氏阴性菌中均发现了反应性物质。据推测，在这种情况下，反应性物质主要是2-酮-3-脱氧辛酸（KDO），一种八碳糖酸，通常与沙门氏菌-大肠杆菌群成员的细胞壁脂多糖相关。在所检测的革兰氏阳性菌和酵母中几乎检测不到反应性物质。以干重百分比表示时，革兰氏阴性菌的反应性物质比革兰氏阳性菌多约8倍。有人提出，在革兰氏阳性细胞和酵母中检测到的少量反应性物质是由KDO以外的化合物引起的。
