Kondo S, Haishima Y, Hisatsune K
Department of Microbiology, School of Pharmaceutical Sciences, Josai University, Saitama.
Microbiol Immunol. 1991;35(8):675-80. doi: 10.1111/j.1348-0421.1991.tb01599.x.
The chemical structure of the 2-keto-3-deoxyoctonate (KDO) region of the lipopolysaccharide (LPS) isolated from O1 V. cholerae NIH 41R (Ogawa) was elucidated by dephosphorylation, periodate oxidation and methylation analysis. Methylation analysis of KDO in the dephosphorylated LPS revealed the presence of 5-O-acetyl-1,2,4,6,7,8-hexa-O-methyl-3-deoxy-octitol and 2-keto-3-deoxy-heptulosonic acid was detected in the methanolysate of the periodate-oxidized and dephosphorylated LPS. These results indicated that the site of binding of KDO to the core oligosaccharide is position C5 as in enteric gram-negative bacterial LPS, while only one molecule of the KDO residue carrying phosphate on position C4 is present in the inner core region of the LPS in contrast to enteric gram-negative bacterial LPS in which one molecule of KDO carrying KDO or KDO2----4KDO disaccharide instead of the phosphate group at position C4 is present in its main chain.
通过去磷酸化、高碘酸盐氧化和甲基化分析，阐明了从霍乱弧菌O1 NIH 41R（小川型）分离的脂多糖（LPS）中2-酮-3-脱氧辛糖酸（KDO）区域的化学结构。去磷酸化LPS中KDO的甲基化分析显示存在5-O-乙酰基-1,2,4,6,7,8-六-O-甲基-3-脱氧辛糖醇，并且在高碘酸盐氧化和去磷酸化LPS的甲醇解产物中检测到2-酮-3-脱氧庚糖酸。这些结果表明，与肠道革兰氏阴性菌LPS一样，KDO与核心寡糖的结合位点是C5位，而与肠道革兰氏阴性菌LPS相反，LPS内核区域仅存在一个在C4位携带磷酸的KDO残基分子，在肠道革兰氏阴性菌LPS的主链中存在一个携带KDO或KDO2----4KDO二糖而非C4位磷酸基团的KDO分子。
