Hickisch B, Kopcanova L
Zentralbl Bakteriol Parasitenkd Infektionskr Hyg. 1977;132(5-6):497-504.
In a model experiment with soil, 2 stages of nitrogen (without additional N and with 200 kg N/ha in the form of ammonia) and 3 N-Serve stages (without inhibitor and with 0.25 per cent and 1 per cent of inhibitor, relative to 200 kg of N/ha) were followed up over a period of 3 and 6 weeks, respectively, with respect to the dynamics of the nitrification process, the saprophytic soil microorganisms, and the autotrophic nitrifiers. In a second experiment, the ammonia form of nitrogen was substituted by the nitrate and nitrite form, respectively. No serious influence on the heterotrophic soil micro-organisms could be detected in any experimental variant; N-Serve was slightly stimulating, the autotrophic nitrifiers were noticeably depressed by N-Serve. The nitrification process could clearly be proved after 3 weeks already; by N-Serve it was remarkably delayed. Nitrite that had been added to the soil was scarcely transformed into nitrate, whereas it was somewhat more ammonified. The high loss of nitrogen in the nitrite variant cannot be caused by denitrification alone. When nitrate was used, the nitrification was not delayed.
在一项土壤模型实验中，分别对两个氮阶段（不添加额外氮以及以氨形式添加200千克氮/公顷）和三个N-Serve阶段（不添加抑制剂以及相对于200千克氮/公顷分别添加0.25%和1%的抑制剂）进行了为期3周和6周的跟踪，研究硝化过程、腐生土壤微生物和自养硝化菌的动态变化。在第二个实验中，分别用硝酸盐和亚硝酸盐形式替代了氨态氮。在任何实验变体中均未检测到对异养土壤微生物有严重影响；N-Serve有轻微促进作用，自养硝化菌受到N-Serve的显著抑制。3周后即可清楚证明硝化过程；N-Serve使其显著延迟。添加到土壤中的亚硝酸盐几乎没有转化为硝酸盐，而是有更多的氨化现象。亚硝酸盐变体中氮的高损失不能仅由反硝化作用引起。当使用硝酸盐时，硝化作用没有延迟。
