Joncas J, Chagnon A, Robert J, Pavilanis V
Can Med Assoc J. 1967 Jun 10;96(23):1526-7.
The authors recently reported the occurrence of an unstable cytopathic effect and a "first passage" interference phenomenon in tissue culture as well as the detection of antigens by immunofluorescence following inoculation of material from patients with infectious mononucleosis.These tissue cultures have since been discovered to be contaminated by a bacterial L-form. The part played by the contaminant in the results obtained has been determined: it can explain only one of the patterns shown by immunofluorescence.The same labile interference phenomenon has been observed by two independent investigators.
作者最近报告了在组织培养中出现的不稳定细胞病变效应和“首次传代”干扰现象，以及接种传染性单核细胞增多症患者的材料后通过免疫荧光检测抗原的情况。此后发现这些组织培养物被一种细菌L型污染。已确定污染物在所得结果中所起的作用：它只能解释免疫荧光显示的一种模式。两位独立的研究人员也观察到了同样的不稳定干扰现象。
