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肌红蛋白与3,3-四亚甲基戊二酸酐的反应。

Reaction of myoglobin with 3,3-tetramethyleneglutaric anhydride.

作者信息

Atassi M Z

出版信息

Biochem J. 1967 Feb;102(2):488-91. doi: 10.1042/bj1020488.

DOI:10.1042/bj1020488
PMID:6029606
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1270270/
Abstract
  1. The extent of reaction of the protein with 3,3-tetramethyleneglutaric anhydride was measured by determining the loss of lysine and the equivalent appearance of 3,3-tetramethyleneglutarimidolysine in the hydrolysate at various time-intervals. Of the 19 lysine residues in sperm-whale myoglobin, three did not react with 3,3-tetramethyleneglutaric anhydride. Also, the N-terminal valine did not react. 2. Slight spectral changes were observed on modification of myoglobin. 3. Electrophoretic changes were great since the derivative was negative throughout the range pH6-8.8. 4. The derivative did not react with antisera to native sperm-whale myoglobin.
摘要
  1. 通过测定不同时间间隔水解产物中赖氨酸的损失以及3,3 - 四亚甲基戊二酰亚胺赖氨酸的等量出现，来测量蛋白质与3,3 - 四亚甲基戊二酸酐的反应程度。抹香鲸肌红蛋白的19个赖氨酸残基中，有3个未与3,3 - 四亚甲基戊二酸酐反应。此外，N - 末端缬氨酸也未反应。2. 肌红蛋白修饰后观察到轻微的光谱变化。3. 电泳变化很大，因为衍生物在pH6 - 8.8的整个范围内都带负电。4. 该衍生物不与抗天然抹香鲸肌红蛋白的抗血清反应。
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9c0/1270270/f8598a1e6fcd/biochemj00746-0116-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9c0/1270270/f8598a1e6fcd/biochemj00746-0116-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9c0/1270270/f8598a1e6fcd/biochemj00746-0116-a.jpg

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Reaction of myoglobin with 3,3-tetramethyleneglutaric anhydride.肌红蛋白与3,3-四亚甲基戊二酸酐的反应。
Biochem J. 1967 Feb;102(2):488-91. doi: 10.1042/bj1020488.
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Immunochemistry of sperm-whale myoglobin. XVII. Conformation and immunochemistry of derivatives modified at lysines 98, 140 and 145 by reaction with 3,3-tetramethyleneglutaric anhydride.抹香鲸肌红蛋白的免疫化学。十七。通过与3,3 - 四亚甲基戊二酸酐反应在赖氨酸98、140和145处修饰的衍生物的构象和免疫化学。
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引用本文的文献

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Biochem J. 1980 Dec 1;191(3):673-80. doi: 10.1042/bj1910673.

本文引用的文献

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A steam distillation apparatus suitable for micro-Kjeldahl analysis.一种适用于微量凯氏定氮分析的水蒸气蒸馏装置。
Biochem J. 1942 Dec;36(10-12):790-1. doi: 10.1042/bj0360790.
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N-ACETYL GROUPS IN VITELLENIN.卵黄磷蛋白中的N-乙酰基团
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ANALYSIS OF THE ACETYL GROUPS IN OVINE LUTEINIZING HORMONE BY GAS CHROMATOGRAPHY.气相色谱法分析绵羊促黄体激素中的乙酰基
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PROPERTIES OF COMPONENTS OF MYOGLOBIN OF THE SPERM WHALE.抹香鲸肌红蛋白成分的特性
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Hemoglobin F1, an acetyl-containing hemoglobin.血红蛋白F1，一种含乙酰基的血红蛋白。
Biochim Biophys Acta. 1962 Oct 8;63:532-4. doi: 10.1016/0006-3002(62)90125-7.
6
A micromethod for the determination of N- and O-acetyl groups.一种测定N-和O-乙酰基的微量方法。
Biochem J. 1963 Mar;86(3):397-401. doi: 10.1042/bj0860397.
7
Carbohydrates in protein. 2. The hexose, hexosamine, acetyl and amide-nitrogen content of hen's-egg albumin.蛋白质中的碳水化合物。2. 鸡蛋白蛋白的己糖、己糖胺、乙酰基和酰胺氮含量。
Biochem J. 1960 Nov;77(2):239-47. doi: 10.1042/bj0770239.
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Isolation of acetylpeptide from enzymic digests of TMV-protein.从烟草花叶病毒蛋白的酶解消化物中分离乙酰肽。
Biochim Biophys Acta. 1958 Apr;28(1):184-91. doi: 10.1016/0006-3002(58)90445-1.
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Starch gel electrophoresis in a discontinous system of buffers.在不连续缓冲系统中的淀粉凝胶电泳。
Nature. 1957 Dec 28;180(4600):1477-9. doi: 10.1038/1801477a0.
10
Role of the amino groups and C-terminal of sperm-whale myoglobin in the antigen-antibody reaction.抹香鲸肌红蛋白的氨基和C末端在抗原-抗体反应中的作用。
Nature. 1966 Mar 19;209(5029):1209-11. doi: 10.1038/2091209a0.