卷心菜叶磷脂酶D对溶血卵磷脂的作用。
The action of cabbage-leaf phospholipase D upon lysolecithin.
作者信息
Long C, Odavić R, Sargent E J
出版信息
Biochem J. 1967 Jan;102(1):216-20. doi: 10.1042/bj1020216.
Abstract
- The action of the water-soluble phospholipase D from Savoy cabbage leaves upon an aqueous solution of lysolecithin has been studied. 2. Optimum conditions required the presence of Ca(2+) ions and pH5.8. 3. Equivalence was found between the amounts of lysolecithin hydrolysed and free choline released. The reaction was not accompanied by deacylation. 4. As the enzymic degradation proceeded, an opalescence developed, owing to the insolubility of the reaction product in the presence of Ca(2+) ions. 5. Evidence has been obtained indicating the heterogeneity of this reaction product.
摘要
- 对皱叶甘蓝叶片中的水溶性磷脂酶D作用于溶血卵磷脂水溶液的情况进行了研究。2. 最适条件要求存在Ca(2+)离子且pH值为5.8。3. 发现水解的溶血卵磷脂量与释放的游离胆碱量相当。该反应未伴随脱酰基作用。4. 随着酶促降解的进行，由于反应产物在Ca(2+)离子存在下不溶，出现了乳光现象。5. 已获得证据表明该反应产物具有异质性。
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