大鼠组织中含硫氨基酸的研究。
A study of the sulphur amino acids of rat tissues.
作者信息
Gaull G E, Gaitonde M K
出版信息
Biochem J. 1967 Jan;102(1):294-303. doi: 10.1042/bj1020294.
Abstract
- In a study of the metabolism of l-[(35)S]methionine in vivo, the labelled sulphur compounds of rat liver and brain were separated first by ion-exchange chromatography into two fractions containing (i) free sulphur amino acids such as methionine, cystathionine, cyst(e)ine and homocyst(e)ine and (ii) glutathione. 2. Two-dimensional paper chromatography with butan-1-ol-acetic acid or propionic acid-water in the first direction and 80% acetone or acetone-ethyl methyl ketone-water in the second direction was found superior to other solvent systems for separating the sulphur amino acids. 3. At 10min. after injection of [(35)S]methionine only a small part of the (35)S was found combined in free methionine or other free sulphur amino acids. 4. Evidence was obtained of the presence of adenosyl[(35)S]methionine and adenosyl[(35)S]homocysteine in perchloric acid extracts of rat liver and brain. 5. The trans-sulphuration pathway was active in brain as well as in liver.
摘要
- 在一项关于L-[(35)S]蛋氨酸体内代谢的研究中，大鼠肝脏和大脑中的标记硫化合物首先通过离子交换色谱法分离成两个部分，其中一部分包含（i）游离硫氨基酸，如蛋氨酸、胱硫醚、胱（硫）氨酸和高胱（硫）氨酸，另一部分包含（ii）谷胱甘肽。2. 发现用1-丁醇-乙酸或丙酸-水在第一方向、80%丙酮或丙酮-甲乙酮-水在第二方向进行二维纸色谱法，在分离硫氨基酸方面优于其他溶剂系统。3. 注射[(35)S]蛋氨酸后10分钟，仅发现一小部分(35)S与游离蛋氨酸或其他游离硫氨基酸结合。4. 在大鼠肝脏和大脑的高氯酸提取物中获得了腺苷[(35)S]蛋氨酸和腺苷[(35)S]高半胱氨酸存在的证据。5. 转硫途径在大脑和肝脏中均有活性。
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