用于同时对牛血浆中转铁蛋白、转铁蛋白后蛋白、白蛋白和白蛋白后蛋白进行表型分析的水平聚丙烯酰胺梯度凝胶电泳
Horizontal polyacrylamide gradient gel electrophoresis for the simultaneous phenotyping of transferrin, post-transferrin, albumin and post-albumin in the blood plasma of cattle.
作者信息
Gahne B, Juneja R K, Grolmus J
出版信息
Anim Blood Groups Biochem Genet. 1977;8(3):127-37. doi: 10.1111/j.1365-2052.1977.tb01637.x.
Abstract
A simple method of horizontal polyacrylamide gel electrophoresis was described for the simultaneous phenotyping of transferrin, post-transferrin, albumin and post-albumin in the blood plasma of cattle. A step gradient gel of 8, 4, 12 and 14% acrylamide concentration was used. The method enabled the detection of a new protein polymorphism in the post-transferrin region. Two alleles were observed. The transferrin phenotypes involving D1 and D2 alleles were clearly separated. The resolution of the post-albumin fractions was also better than described by earlier methods.
摘要
本文描述了一种简单的水平聚丙烯酰胺凝胶电泳方法，用于同时对牛血浆中的转铁蛋白、后转铁蛋白、白蛋白和后白蛋白进行表型分析。使用了浓度为8%、4%、12%和14%的梯度凝胶。该方法能够检测后转铁蛋白区域的一种新的蛋白质多态性。观察到两个等位基因。涉及D1和D2等位基因的转铁蛋白表型被清晰分离。后白蛋白组分的分辨率也比早期方法所描述的更好。
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