家兔（穴兔）中Es-1酯酶的异质性。

Heterogeneity of Es-1 esterases in the rabbit (Oryctolagus cuniculus).

作者信息

Bellen H, Van de Weghe A, Bouquet Y, Van Zutphen L F

出版信息

Biochem Genet. 1984 Oct;22(9-10):853-70. doi: 10.1007/BF00499477.

Abstract

Analysis of the Es-1 system in the rabbit with polyacrylamide gel electrophoresis (PAGE) revealed a high degree of individual variation. In the liver the number of esterase bands found in the Es-1 region of the gels ranged from 2 to 16. The results indicate that one locus with three alleles is responsible for all of the esterase bands in the Es-1 region. The most plausible explanation for the observed heterogeneity is that each of the alleles codes for a protein (MW 65,000 +/- 2000) that is changed by posttranslational modifications, thus giving rise to two to five monomeric enzymes with esterase activity. Polymerization of these monomers then results in 1-11 dimers. Based on similarities with mouse Es-9, chromosomal homology between rabbit Es-1 and mouse Es-9 is proposed.

摘要

用聚丙烯酰胺凝胶电泳（PAGE）分析兔的Es-1系统，发现个体差异程度很高。在肝脏中，凝胶Es-1区域发现的酯酶条带数量在2到16条之间。结果表明，一个具有三个等位基因的基因座负责Es-1区域的所有酯酶条带。对观察到的异质性最合理的解释是，每个等位基因编码一种蛋白质（分子量65,000±2000），该蛋白质通过翻译后修饰发生变化，从而产生两到五种具有酯酶活性的单体酶。这些单体的聚合然后产生1-11种二聚体。基于与小鼠Es-9的相似性，提出了兔Es-1和小鼠Es-9之间的染色体同源性。