Schlüns J, Graf R
Histochemistry. 1976 Aug 25;48(3):219-32. doi: 10.1007/BF00497457.
We studied the distribution of endogenous peroxidase in the spleen of swine by modifications of the Graham and Karnovsky diaminobenzidine procedure. There is a peroxidatic activity in the majority of the ellipsoid cells (cells of the sheathed capillaries of Schweigger-Seidel), which is localized in the endoplasmic reticulum and the perinuclear cisterna. This staining is inhibited completely by aminotriazole and is rapidly destroyed even by low concentratoins of glutaraldehyde. Furthermore, the reaction is abolished after boiling of tissue sections or in the absence of H2O2. The macrophages of the red pulp and a minority of the ellipsoid cells are peroxidase negative. Our results are discussed in respect to some recent studies on the system of mononuclear phagocytes. It is suggested, that the enzyme active ellipsoid cells represent a special form of macrophages, enzyme histochemically related to Kupffer cells and resident peritoneal macrophages. The enzyme negative cells of the ellipsoids are probably fibroblasts.
我们通过对格雷厄姆和卡诺夫斯基二氨基联苯胺法进行改进，研究了猪脾脏内源性过氧化物酶的分布情况。大多数椭球细胞（施魏格 - 赛德尔被膜毛细血管的细胞）存在过氧化物酶活性，其定位于内质网和核周池。这种染色可被氨基三唑完全抑制，即使是低浓度的戊二醛也能迅速将其破坏。此外，组织切片煮沸后或在没有过氧化氢的情况下，反应会消失。红髓中的巨噬细胞和少数椭球细胞过氧化物酶呈阴性。我们结合近期关于单核吞噬细胞系统的一些研究对结果进行了讨论。有人提出，具有酶活性的椭球细胞代表了巨噬细胞的一种特殊形式，在酶组织化学上与库普弗细胞和常驻腹膜巨噬细胞相关。椭球细胞中酶阴性的细胞可能是成纤维细胞。
