Bodel P T, Nichols B A, Bainton D F
Am J Pathol. 1978 Apr;91(1):107-17.
Unlike resident peritoneal macrophages, which contain peroxidase in the rough endoplasmic reticulum (RER) and perinuclear cisternae (PN), macrophages elicited into the rabbit peritoneal cavity by various stimulants lack the enzyme. Since we had previously found that such peroxidase reactivity rapidly appears in the RER and PN of blood monocytes after surface adherence in vitro, we wondered whether the enzyme could be similarly produced in elicited macrophages by adherence. Cells from peritoneal exudates (96 hours after endotoxin injection) were harvested, suspended in culture medium, and allowed to adhere to fibrin-coated or plastic surfaces. Following culture for various intervals, they were fixed, incubated for peroxidase, and examined by electron microscopy. We observed that these elicited cells, which initially contained no cytochemically detectable peroxidase, acquired peroxidatic activity in the RER and PN within 2 hours after adherence in culture. Thus macrophages, like blood monocytes, may rapidly acquire peroxidase reactivity as a consequence of plasma membrane: external surface interaction. In view of this finding, it would seem unwise to use peroxidase localization as the basis for advocating the existence of two separate lines of peritoneal macrophages, as has been proposed by previous investigators.
与驻留腹膜巨噬细胞不同，驻留腹膜巨噬细胞在粗面内质网（RER）和核周池（PN）中含有过氧化物酶，而通过各种刺激物诱导进入兔腹腔的巨噬细胞则缺乏这种酶。由于我们之前发现，体外表面黏附后，血液单核细胞的RER和PN中会迅速出现这种过氧化物酶反应性，我们想知道这种酶是否能通过黏附在诱导巨噬细胞中类似地产生。收集来自腹膜渗出液的细胞（内毒素注射后96小时），悬浮于培养基中，并使其黏附于纤维蛋白包被的或塑料表面。在培养不同时间间隔后，将细胞固定，进行过氧化物酶孵育，并通过电子显微镜检查。我们观察到，这些最初不含细胞化学可检测过氧化物酶的诱导细胞，在培养中黏附后2小时内，RER和PN中获得了过氧化物酶活性。因此，巨噬细胞与血液单核细胞一样，可能由于质膜与外表面的相互作用而迅速获得过氧化物酶反应性。鉴于这一发现，像之前研究者所提议的那样，将过氧化物酶定位作为主张存在两种不同腹膜巨噬细胞系的依据似乎并不明智。
