用于研究大麦糊粉层中α-淀粉酶合成的无细胞体系。
A cell-free system for the study of alpha-amylase synthesis in barley aleurone layers.
作者信息
Duffus J H
出版信息
Biochem J. 1967 Apr;103(1):215-7. doi: 10.1042/bj1030215.
Abstract
- A cell-free system capable of alpha-amylase synthesis has been obtained from the aleurone layers of germinating barley. 2. This system requires potassium chloride, sucrose and an amino acid mixture in order to function. The crude preparation does not require calcium chloride. Chloramphenicol inhibits alpha-amylase synthesis as indicated both by increase in measurable enzyme activity and incorporation of l-[U-(14)C]glutamic acid.
摘要
- 已从发芽大麦的糊粉层获得了一种能够合成α-淀粉酶的无细胞体系。2. 该体系为发挥作用需要氯化钾、蔗糖和一种氨基酸混合物。粗制品不需要氯化钙。氯霉素抑制α-淀粉酶的合成，这可通过可测量的酶活性增加以及l-[U-(14)C]谷氨酸的掺入得以表明。
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本文引用的文献
1
Gibberellic Acid Controlled Synthesis of alpha-Amylase in Barley Endosperm.赤霉素对大麦胚乳中α-淀粉酶合成的调控
Plant Physiol. 1964 May;39(3):413-5. doi: 10.1104/pp.39.3.413.
2
Physiological Effects of Gibberellic Acid: I. On Carbohydrate Metabolism and Amylase Activity of Barley Endosperm.赤霉素的生理效应：I. 对大麦胚乳碳水化合物代谢和淀粉酶活性的影响
Plant Physiol. 1960 May;35(3):293-9. doi: 10.1104/pp.35.3.293.
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HORMONAL CONTROL OF ENZYME SYNTHESIS IN BARLEY ENDOSPERM.大麦胚乳中酶合成的激素调控
Proc Natl Acad Sci U S A. 1964 Jul;52(1):100-6. doi: 10.1073/pnas.52.1.100.
4
The glycogen-amylase complex as a means of obtaining highly purified alpha-amylases.糖原 - 淀粉酶复合物作为获得高度纯化α - 淀粉酶的一种手段。
Biochim Biophys Acta. 1962 Dec 4;65:200-6. doi: 10.1016/0006-3002(62)91039-9.
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Induction of alkaline phosphatase in a subcellular preparation from Escherichia coli.大肠杆菌亚细胞制剂中碱性磷酸酶的诱导
Biochem J. 1964 Feb;90(2):378-91. doi: 10.1042/bj0900378.
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