癌症中的酶。来自鸡肝的天冬酰胺酶。
Enzymes in cancer. Asparaginase from chicken liver.
作者信息
Ohnuma T, Bergel F, Bray R C
出版信息
Biochem J. 1967 Apr;103(1):238-45. doi: 10.1042/bj1030238.
Abstract
- A procedure for partial purification of asparaginase from chicken liver is presented. 2. The bulk of the enzyme is located in the soluble fraction of chicken liver. 3. Molecular weights of chicken-liver asparaginase and of the guinea-pig serum enzyme, estimated by gel filtration, were 306000 and 210000 respectively. The Michaelis constants (K(m)) at 37 degrees and pH8.5 were 6.0x10(-5)m and 7.2x10(-5)m respectively. 4. At 50 degrees the chicken-liver enzyme was moderately stable, some activity being lost by aggregation; in dilute electrolyte solutions the activity rapidly diminished. 5. The anti-lymphoma effect of guinea-pig serum in mice carrying the 6C3HED tumour was confirmed. Chicken-liver asparaginase also showed an effect but in this case the enzyme preparation had to be administered repeatedly. 6. Guinea-pig serum asparaginase was stable for several days in mouse blood, after intraperitoneal injection, whereas chicken-liver asparaginase rapidly disappeared. 7. Aspartic acid beta-hydrazide was shown to be a competitive inhibitor of chicken-liver asparaginase with K(i) approx. 5.6x10(-4)m. In mice it produced an anti-lymphoma effect, as reported previously.
摘要
- 本文介绍了一种从鸡肝中部分纯化天冬酰胺酶的方法。2. 该酶的大部分存在于鸡肝的可溶部分。3. 通过凝胶过滤法估算，鸡肝天冬酰胺酶和豚鼠血清酶的分子量分别为306000和210000。在37摄氏度和pH8.5条件下，它们的米氏常数（K(m)）分别为6.0×10(-5)m和7.2×10(-5)m。4. 在50摄氏度时，鸡肝酶具有一定稳定性，部分活性因聚集而丧失；在稀电解质溶液中，活性迅速降低。5. 证实了豚鼠血清对携带6C3HED肿瘤的小鼠具有抗淋巴瘤作用。鸡肝天冬酰胺酶也显示出一定作用，但在这种情况下，酶制剂必须反复给药。6. 腹腔注射后，豚鼠血清天冬酰胺酶在小鼠血液中可稳定存在数天，而鸡肝天冬酰胺酶则迅速消失。7. 天冬氨酸β-酰肼被证明是鸡肝天冬酰胺酶的竞争性抑制剂，其抑制常数（K(i)）约为5.6×10(-4)m。如先前报道，它在小鼠体内产生了抗淋巴瘤作用。
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