大鼠肝脏和骨骼肌核糖体亚基的可逆解离与活性
The reverible dissociation and activity ofribosoma subunits of liver and skeletal muscle from rats.
作者信息
von der Decke A, Ashby P, McIlreavy D, Campbell P N
出版信息
Biochem J. 1970 Dec;120(4):815-9. doi: 10.1042/bj1200815.
Abstract
Ribosomal subunits were prepared from rat liver and skeletal muscle after incubation with puromycin and treatment at low concentrations of Mg(2+). After isolation the resulting subunits could be recombined and the particles effected the synthesis of polyphenylalanine in the presence of polyuridylic acid. Hybrid particles formed from subunits of liver and muscle respectively were also active. The homogeneity of the isolated subunits was checked by polyacrylamide-gel-agarose electrophoresis. The method is shown to be a reliable and comparatively simple way of preparing active ribosomal subunits from skeletal muscle.
摘要
用嘌呤霉素孵育并在低浓度镁离子（Mg²⁺）条件下处理后，从大鼠肝脏和骨骼肌中制备核糖体亚基。分离后，所得的亚基可以重新组合，并且这些颗粒在多聚尿苷酸存在的情况下能够合成聚苯丙氨酸。分别由肝脏和肌肉的亚基形成的杂交颗粒也具有活性。通过聚丙烯酰胺 - 琼脂糖凝胶电泳检查分离的亚基的均一性。结果表明，该方法是从骨骼肌制备活性核糖体亚基的一种可靠且相对简单的方法。
相似文献
1
The reverible dissociation and activity ofribosoma subunits of liver and skeletal muscle from rats.大鼠肝脏和骨骼肌核糖体亚基的可逆解离与活性
Biochem J. 1970 Dec;120(4):815-9. doi: 10.1042/bj1200815.
2
Ribosomal subunits from rat liver. Isolation and recombination into active ribosomal particles.来自大鼠肝脏的核糖体亚基。分离并重组为活性核糖体颗粒。
Ital J Biochem. 1970 Nov-Dec;19(6):397-416.
3
Ribosomal subunits from rat liver. 1. Isolation and properties of active 40-S and 60-S subunits.来自大鼠肝脏的核糖体亚基。1. 活性40-S和60-S亚基的分离及特性
Eur J Biochem. 1972 Apr 11;26(3):347-53. doi: 10.1111/j.1432-1033.1972.tb01773.x.
4
A decreased aminoacyl-transfer-ribonucleic acid-binding capacity of 40S ribosomal subunits resulting from hypophysectomy of the rat.大鼠垂体切除导致40S核糖体亚基的氨酰转移核糖核酸结合能力下降。
Biochem J. 1972 Apr;127(2):411-7. doi: 10.1042/bj1270411.
5
Studies on a nucleoprotein prepared from rat liver polysomes by digestion with T1 ribonuclease.关于用T1核糖核酸酶消化大鼠肝脏多核糖体所制备的一种核蛋白的研究。
Biochem J. 1969 Jul;113(4):643-50. doi: 10.1042/bj1130643.
6
Protein synthesis by membrane-bound and free ribosomes of secretory and non-secretory tissues.分泌性和非分泌性组织的膜结合核糖体和游离核糖体的蛋白质合成。
Biochem J. 1971 Feb;121(4):683-94. doi: 10.1042/bj1210683.
7
Comparison of the effect of various methods of dissociation on the protein composition of rat liver ribosomal subunits.不同解离方法对大鼠肝脏核糖体亚基蛋白质组成影响的比较
Can J Biochem. 1975 Sep;53(9):935-42. doi: 10.1139/o75-130.
8
Initiation of polyphenylalanine synthesis by N-acetylphenylalanyl-SRNA.由N-乙酰苯丙氨酰-SRNA引发聚苯丙氨酸的合成。
Proc Natl Acad Sci U S A. 1967 Apr;57(4):1050-7. doi: 10.1073/pnas.57.4.1050.
9
[Accumulation of aminoacyl-tRNA in rat liver ribosomes].[大鼠肝脏核糖体中氨酰 - tRNA的积累]
Z Naturforsch B. 1967 Dec;22(12):1312-8.
10
A comparison of the proteins of rat skeletal muscle and liver ribosomes by two-dimensional polyacrylamide gel electrophoresis. Observations on the partition of proteins between ribosomal subunits and a description of two acidic proteins in the large subunit.通过二维聚丙烯酰胺凝胶电泳对大鼠骨骼肌和肝脏核糖体的蛋白质进行比较。关于核糖体亚基间蛋白质分配的观察以及对大亚基中两种酸性蛋白质的描述。
J Biol Chem. 1974 Apr 10;249(7):2258-67.
引用本文的文献
1
The distribution of ribosomes between different functional states in livers of fed and starved mice.喂食和饥饿小鼠肝脏中核糖体在不同功能状态之间的分布。
Biochem J. 1973 Jun;134(2):387-98. doi: 10.1042/bj1340387.
2
Dissociation of ribosomes from oocytes of Xenopus laevis into active subparticles.非洲爪蟾卵母细胞中的核糖体解离为活性亚颗粒。
Biochem J. 1971 Oct;124(5):897-903. doi: 10.1042/bj1240897.
3
The protein-synthesizing activity of ribosomes isolated from the mammary gland of lactating and pregnant guinea pigs.从哺乳期和怀孕豚鼠乳腺中分离出的核糖体的蛋白质合成活性。
Biochem J. 1971 Aug;123(5):865-74. doi: 10.1042/bj1230865.
4
Inhibition of translation in liver polyribosomes by a new substituted thiopseudourea with antitumour action.一种具有抗肿瘤作用的新型取代硫代假脲对肝脏多核糖体翻译的抑制作用。
Biochem J. 1974 Feb;138(2):129-41. doi: 10.1042/bj1380129.
5
The ribonucleic acids of the mammary gland of the guinea pig.豚鼠乳腺的核糖核酸
Biochem J. 1975 Mar;146(3):575-83. doi: 10.1042/bj1460575.
本文引用的文献
1
Ribosomal aggregate engaged in protein synthesis: characterization of the ergosome.参与蛋白质合成的核糖体聚集体：多核蛋白体的表征
Nature. 1963 Feb 2;197:430-5. doi: 10.1038/197430a0.
2
Protein synthesis by microsomal particles from regenerating rat liver.来自再生大鼠肝脏的微粒体颗粒的蛋白质合成
Biochem J. 1967 Apr;103(1):280-8. doi: 10.1042/bj1030280.
3
Active hybrid 80 s particles formed from subunits of rat, rabbit and protozoan (Tetrahymena pyriformis) ribosomes.由大鼠、兔子和原生动物（梨形四膜虫）核糖体亚基形成的活性杂交80s颗粒。
J Mol Biol. 1969 Jul 14;43(1):151-61. doi: 10.1016/0022-2836(69)90085-0.
4
Protein feeding of rats after protein starvation: Incorporation of amino acid into polypeptide by skeletal muscle polyribosomes.蛋白质饥饿后对大鼠进行蛋白质喂养：氨基酸通过骨骼肌多核糖体掺入多肽。
J Nutr. 1970 Jun;100(6):623-30. doi: 10.1093/jn/100.6.623.
5
6
The effect of incubation with puromycin on the dissociation of rat liver ribosomes into active subunits.嘌呤霉素孵育对大鼠肝脏核糖体解离为活性亚基的影响。
Biochem Biophys Res Commun. 1969 Sep 24;37(1):143-50. doi: 10.1016/0006-291x(69)90892-4.
7
Formation of active hybrids from subunits of muscle ribosomes from normal and diabetic rats.正常和糖尿病大鼠肌肉核糖体亚基形成活性杂种。
Proc Natl Acad Sci U S A. 1968 Jun;60(2):569-74. doi: 10.1073/pnas.60.2.569.
8
Dissociation and reassociation of skeletal muscle ribosomes.骨骼肌核糖体的解离与重新结合。
J Mol Biol. 1969 Jul 14;43(1):135-49. doi: 10.1016/0022-2836(69)90084-9.
9
Electrophoretic characterization of bacterial polyribosomes in agarose-acrylamide composite gels.细菌多核糖体在琼脂糖-丙烯酰胺复合凝胶中的电泳表征
J Mol Biol. 1969 Apr 14;41(1):139-47. doi: 10.1016/0022-2836(69)90131-4.
10
Biosynthesis of microsomal nicotinamide-adenine dinucleotide phosphate-cytochrome c reductase by membrane-bound and free polysomes from rat liver.大鼠肝脏中膜结合型和游离多聚核糖体对微粒体烟酰胺腺嘌呤二核苷酸磷酸 - 细胞色素c还原酶的生物合成
Biochem J. 1969 Apr;112(2):139-47. doi: 10.1042/bj1120139.
Zotero 插件