Austen R A, Dunn N W
Aust J Biol Sci. 1977 Aug;30(4):357-66. doi: 10.1071/bi9770357.
A comparative study of the NAH and TOL catabolic plasmids was carried out to provide information for future genetic manipulation experiments involving these two plasmids. The plasmids were studied in a strain of P. putida and its mutant derivatives. The NAH and TOL plasmids were found to be incompatible. Under the conditions used in these experiments the TOL plasmid transferred into some strains into which NAH was unable to transfer. The use of mutants to remove certain catabolic activities encoded by the bacterial host cell facilitated the allocation of growth genotypes to the NAH and TOL plasmids. TOL encoded the degradation of benzoate, m-toluate and p-toluate, whereas NAH encoded the degradation of naphthalene and salicylate. The other plasmid-associated growth phenotypes were partly plasmid-specified and partly specified by the host cell. The pH optimum of the catechol 2,3-dioxygenase specified by the TOL plasmid was approximately 6.7, whereas that of the NAH-encoded enzyme was approximately 8.3.
对NAH和TOL分解代谢质粒进行了比较研究，以便为今后涉及这两种质粒的基因操作实验提供信息。在恶臭假单胞菌菌株及其突变衍生物中对这些质粒进行了研究。发现NAH和TOL质粒是不相容的。在这些实验所使用的条件下，TOL质粒转移到了一些NAH无法转移到的菌株中。利用突变体去除细菌宿主细胞编码的某些分解代谢活性，有助于将生长基因型分配给NAH和TOL质粒。TOL编码苯甲酸、间甲苯酸和对甲苯酸的降解，而NAH编码萘和水杨酸的降解。其他与质粒相关的生长表型部分由质粒决定，部分由宿主细胞决定。TOL质粒所编码的儿茶酚2,3-双加氧酶的最适pH约为6.7，而NAH编码的酶的最适pH约为8.3。
