Matcham G W, Dodgson K S
Biochem J. 1977 Dec 1;167(3):717-22. doi: 10.1042/bj1670717.
During the course of the purification of novel stereospecific secondary aklylsulphohydrolases present in certain detergent-degrading micro-organisms, it became apparent that substrates prepared by sulphating secondary alcohols with H2SO4 are heterogeneous. Apart from the racemization that occurs if resolved alcohols are sulphated, evidence is provided to show that other isomers are produced in which the position of the ester sulphate group on the alkyl chain has been altered. These changes can be avoided if pyridine/SO3 reagent (prepared with SO3) is substituted as sulphating agent. Experiments in which secondary alkyl sulphates prepared by both methods were tested as potential substrates for the two secondary alkylsulphohydrolase enzymes of Comamonas terrigena have provided initial information about the specificity of the enzymes.
在纯化某些降解洗涤剂的微生物中存在的新型立体特异性仲烷基硫醇水解酶的过程中，很明显，用H2SO4硫酸化仲醇制备的底物是异质的。除了拆分后的醇被硫酸化时发生的外消旋作用外，还提供了证据表明会产生其他异构体，其中烷基链上硫酸酯基团的位置发生了改变。如果用吡啶/SO3试剂（用SO3制备）替代作为硫酸化剂，则可以避免这些变化。用两种方法制备的仲烷基硫酸盐作为土生丛毛单胞菌的两种仲烷基硫醇水解酶的潜在底物进行测试的实验，提供了有关这些酶特异性的初步信息。
