Sundström C, Nilsson K
Br J Haematol. 1977 Dec;37(4):489-501. doi: 10.1111/j.1365-2141.1977.tb01022.x.
Twenty-three human haematopoietic cell lines, normal and mitogen stimulated peripheral blood lymphocytes and tumour material from fresh leukaemias, myelomas and lymphomas were investigated with a panel of cytochemical reactions. Normal and mitogen stimulated lymphocytes, non-neoplastic lymphoblastoid cell lines (LCL), lymphoma lines with B-lymphocyte characteristics, chronic lymphocytic leukaemia and fresh lymphocytic lymphomas reacted weakly or negatively with all stains. T-lymphocyte acute leukaemia lines were PAS and alpha-naphtyl acetate esterase positive. Myeloma lines and fresh myelomas were strongly beta-glucoronidase positive. A histiocytic lymphoma cell line was strongly esterase positive with naphtol AS-D acetate esterase inhibited by NaF. The three fresh histiocytic lymphomas, however, reacted as the lymphocytic lymphomas suggesting a lymphoid origin. A myeloid leukaemia line was strongly positive for acid phsophatase. No major disagreement was noted between the reactivity of established neoplastic lines and the corresponding fresh biopsy cells indicating an unaltered qualitative expression of enzyme production after prolonged in vitro culture.
采用一组细胞化学反应对23个人类造血细胞系、正常及经有丝分裂原刺激的外周血淋巴细胞以及来自新鲜白血病、骨髓瘤和淋巴瘤的肿瘤材料进行了研究。正常及经有丝分裂原刺激的淋巴细胞、非肿瘤性淋巴母细胞系（LCL）、具有B淋巴细胞特征的淋巴瘤系、慢性淋巴细胞白血病及新鲜淋巴细胞淋巴瘤对所有染色反应均较弱或呈阴性。T淋巴细胞急性白血病系PAS和α-萘乙酸酯酶呈阳性。骨髓瘤系及新鲜骨髓瘤β-葡萄糖醛酸酶呈强阳性。1个组织细胞淋巴瘤细胞系酯酶呈强阳性，萘酚AS-D乙酸酯酶被NaF抑制。然而，3例新鲜组织细胞淋巴瘤的反应与淋巴细胞淋巴瘤相同，提示其起源于淋巴样细胞。1个髓细胞白血病系酸性磷酸酶呈强阳性。已建立的肿瘤细胞系与相应新鲜活检细胞的反应性之间未发现重大差异，表明长期体外培养后酶产生的定性表达未改变。
