Beck F, Lloyd J B, Griffiths A
Science. 1967 Sep 8;157(3793):1180-2. doi: 10.1126/science.157.3793.1180.
A mechanism of mammalian teratogenesis involving inhibition of embryotrophic nutrition is suggested and exemplified by the action of trypan blue on pregnant rats. There is evidence for the localization of trypan blue in heterolysosomes of the epithelium of the visceral yolk sac, and our experiments indicate that the dye is an inhibitor of a selection of hydrolytic enzymes present in lysosomal fractions from homogenates of rat visceral yolk sac. It seems likely that trypan blue inhibits the intracellular digestion of embryotroph by the visceral yolk-sac epithelium; the conceptus may therefore be deprived of essential nutrients at critical stages of development.
有人提出一种涉及抑制胚胎营养的哺乳动物致畸机制，并以锥虫蓝对怀孕大鼠的作用为例进行了说明。有证据表明锥虫蓝定位于内脏卵黄囊上皮的异溶酶体中，我们的实验表明，这种染料是大鼠内脏卵黄囊匀浆溶酶体组分中某些水解酶的抑制剂。锥虫蓝似乎抑制了内脏卵黄囊上皮对胚胎营养物质的细胞内消化；因此，在发育的关键阶段，胚胎可能会被剥夺必需的营养物质。
