硫胺素缺乏、吡硫胺素和氧硫胺素对大鼠肝脏和脑内丙酮酸代谢的体内影响。
Effect of thiamine deficiency, pyrithiamine and oxythiamine on pyruvate metabolism in rat liver and brain in vivo.
作者信息
Meghal S K, O'Neal R M, Koeppe R E
出版信息
J Nutr Sci Vitaminol (Tokyo). 1977;23(5):385-93. doi: 10.3177/jnsv.23.385.
Rats were fed either a thiamine-deficient diet of diets containing pyrithiamine or oxythiamine. When symptoms of thiamine deficiency appeared, the animals were injected intraperitoneally with [2-14C] pyruvate six to twelve minutes prior to sacrifice. Free glutamic and aspartic acids were isolated from liver and brain and degraded. The results indicate that, in thiamine-deficient or oxythiamine-treated rats, pyruvate metabolism in liver and brain is similar to that in normal animals. In contrast, pyrithiamine drastically decreases the oxidative decarboxylation of pyruvate by rat liver.
给大鼠喂食缺乏硫胺素的饮食，或含有吡硫胺素或氧硫胺素的饮食。当出现硫胺素缺乏症状时，在处死动物前6至12分钟，给它们腹腔注射[2-¹⁴C]丙酮酸。从肝脏和大脑中分离出游离的谷氨酸和天冬氨酸并进行降解。结果表明，在硫胺素缺乏或经氧硫胺素处理的大鼠中，肝脏和大脑中的丙酮酸代谢与正常动物相似。相比之下，吡硫胺素会大幅降低大鼠肝脏中丙酮酸的氧化脱羧作用。
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