Sullivan T J, Parker K L, Kulczycki A, Parker C W
J Immunol. 1976 Sep;117(3):713-6.
Changes in rat mast cell cyclic adenosine 3',5' monophosphate (cAMP) concentrations during stimulation of histamine release by concanavalin A (con A) and anti-IgE were studied. Con A caused an increase in cAMP with a mean peak level at 20 sec of 232% of control (range 164% to 365%). Con A-stimulated cells demonstrated falls toward control levels after 20 sec, but generally remained above control for at least 5 min. By 10 min cAMP had returned to control values. The con A effect on cAMP occurred in the absence of phosphatidyl serine but was markedly inhibited by 5 mM alpha-methyl-D-mannose. Anti-IgE induced a less marked increase in cAMP (157% of control, range 110% to 540% of control) which reached a peak at 20 sec. Two monospecific goat anti-rat myeloma IgE antisera induced similar changes in cAMP whereas normal goat IgG had no effect. These peak values were followed by a rapid decrease in cAMP. Within 2 min the cAMP content of anti-IgE stimulated cells had fallen to levels well below control and remained below control levels from 45 sec to over 15 min. Histamine release in both systems began after the peak cAMP levels, during the period of rapid destruction of cAMP.
研究了伴刀豆球蛋白A（Con A）和抗IgE刺激组胺释放过程中大鼠肥大细胞环磷酸腺苷（cAMP）浓度的变化。Con A使cAMP增加，平均峰值水平在20秒时为对照的232%（范围为164%至365%）。Con A刺激的细胞在20秒后向对照水平下降，但通常在至少5分钟内保持高于对照水平。到10分钟时，cAMP已恢复到对照值。Con A对cAMP的作用在不存在磷脂酰丝氨酸的情况下发生，但被5 mMα-甲基-D-甘露糖显著抑制。抗IgE诱导cAMP的增加不太明显（为对照的157%，范围为对照的110%至540%），在20秒时达到峰值。两种单特异性山羊抗大鼠骨髓瘤IgE抗血清诱导cAMP发生类似变化，而正常山羊IgG则无作用。这些峰值之后cAMP迅速下降。在2分钟内，抗IgE刺激细胞的cAMP含量已降至远低于对照的水平，并在45秒至超过15分钟的时间内保持低于对照水平。两个系统中的组胺释放均在cAMP峰值水平之后、cAMP快速破坏期间开始。
