Inagaki N, Kawasaki H, Nagai H
Department of Pharmacology, Gifu Pharmaceutical University, Japan.
Inflamm Res. 1995 Dec;44(12):541-7. doi: 10.1007/BF01757359.
Histamine release from purified rat peritoneal mast cells (PMC) was examined and compared to that from a non-purified preparation (PEC). Both PEC and PMC released similar amounts of histamine upon stimulation with compound 48/80, calcium ionophore A23187 and substance P. In contrast, IgE-dependent histamine release from PMC caused by antigen, anti-IgE and concanavalin A was very low compared to that of PEC. The reduced IgE-dependent histamine release from PMC, however, was recovered when PMC was reconstituted with non-mast cells (NMC) present in the peritoneal cavity. The effect was time-dependent and reached a plateau in 30 min. NMC from both sensitized and non-sensitized rats recovered the reduced histamine release from PMC dose-dependently. The potentiating effect of NMC was observed even in the presence of excess amount of phosphatidylserine. Supernatants of NMC and a mixture of PMC and NMC incubated for 1 hr at 37 degrees C, however, failed to potentiate the histamine release. These results demonstrate that IgE-dependent histamine release from rat peritoneal mast cells is upregulated by other cells present in the peritoneal cavity, and that the mechanism involved is distinct from that of phosphatidylserine.
研究了从纯化的大鼠腹膜肥大细胞（PMC）中组胺的释放情况，并与未纯化制剂（PEC）中的组胺释放进行了比较。在用化合物48/80、钙离子载体A23187和P物质刺激后，PEC和PMC释放的组胺量相似。相比之下，与PEC相比，由抗原、抗IgE和伴刀豆球蛋白A引起的PMC中IgE依赖性组胺释放非常低。然而，当用腹膜腔中存在的非肥大细胞（NMC）重构PMC时，PMC中降低的IgE依赖性组胺释放得以恢复。这种作用是时间依赖性的，30分钟内达到平台期。来自致敏和未致敏大鼠的NMC均剂量依赖性地恢复了PMC中降低的组胺释放。即使存在过量的磷脂酰丝氨酸，也能观察到NMC的增强作用。然而，NMC的上清液以及在37℃孵育1小时的PMC和NMC混合物未能增强组胺释放。这些结果表明，大鼠腹膜肥大细胞中IgE依赖性组胺释放受到腹膜腔中其他细胞的上调，并且所涉及的机制与磷脂酰丝氨酸的机制不同。
