Bernstine E G, Koyama H, Ephrussi B
Somatic Cell Genet. 1977 Mar;3(2):217-25. doi: 10.1007/BF01551816.
Three independent hybrid cell lines were isolated from the fusion of clonal lines of embryonal carcinoma and neuroblastoma. A series of subclones was subsequently derived from the original hybrid clones. In early hybrid generations all hybrid lines showed enhancement of alkaline phosphatase activity, expressing 2--8 times the activity of the teratoma parental line. The overexpression of APase appears to take place in the stationary phase of the growth cycle. Segregation for very high levels of APase activity was observed among subclones of one hybrid line. Specific activities of the segregants ranged from 0.1 to 133. Results of heat denaturation studies are consistent with the hypothesis that it is the embryonal carcinoma APase that is being expressed in the hybrids.
从胚胎癌和神经母细胞瘤的克隆系融合中分离出三个独立的杂交细胞系。随后从原始杂交克隆中获得了一系列亚克隆。在杂交早期世代，所有杂交系都表现出碱性磷酸酶活性增强，其活性是畸胎瘤亲代系的2至8倍。碱性磷酸酶的过表达似乎发生在生长周期的静止期。在一个杂交系的亚克隆中观察到了极高水平碱性磷酸酶活性的分离。分离株的比活性范围为0.1至133。热变性研究结果与以下假设一致，即在杂交体中表达的是胚胎癌碱性磷酸酶。
