Miller R A, Ruddle F H
Somatic Cell Genet. 1977 May;3(3):247-61. doi: 10.1007/BF01538744.
A series of teratocarcinoma-thymus hybrid cells (PCT hybrids), which had been shown previously to give rise to multidifferentiated tumors and hence to be pluripotent, was tested to see whether these cells resembled their embryonal carcinoma parent in other ways as well. PCT hybrid cells looked like embryonal carcinoma parent in other ways as well. PCT hybrid cells looked like embryonal carcinoma cells by phase contrast and electron microscopy, have high levels of alkaline phosphatase, and fail to express Thy 1 alloantigen (which is present on thymocyte parental cells, but not on embryonal carcinoma cells). PCT hybrids do, however, exhibit H2 antigens, which are present only at very low levels, if at all, on embryonal carcinoma cells.
先前已证明一系列畸胎癌-胸腺杂交细胞（PCT杂交细胞）能产生多分化肿瘤，因此具有多能性，现对其进行检测，以确定这些细胞在其他方面是否也与其胚胎癌细胞亲本相似。PCT杂交细胞在其他方面也与胚胎癌细胞亲本相似。通过相差显微镜和电子显微镜观察，PCT杂交细胞看起来像胚胎癌细胞，碱性磷酸酶水平高，且不表达Thy 1同种异体抗原（该抗原存在于胸腺细胞亲本细胞上，但不存在于胚胎癌细胞上）。然而，PCT杂交细胞确实表现出H2抗原，而H2抗原在胚胎癌细胞上即便有表达，水平也非常低。
