Goldrick R B
J Lipid Res. 1967 Nov;8(6):581-8.
Isolated fat cells were used for the study of in vitro effects of insulin on glucose metabolism in human and rat adipose tissue. In human subcutaneous fat cells, effects of insulin could be detected at concentrations of glucose in the medium from 1 to 10 micro moles/ml. Cellular responsiveness was inversely proportional to the glucose level. At a constant concentration of 6 micro moles of glucose per ml, the effects of insulin at various concentrations up to 500 micro U/ml were investigated. At the highest concentration, which gave the maximal response, there was a 100% increase in the conversion of glucose-U-(14)C to glyceride-glycerol and a 40% increase in glucose oxidation. The dose-response curve was steepest between 2 and 20 micro U/ml. Rat epididymal fat cells were much more responsive to insulin. Glucose lipogenesis and pentose cycle activity could also be demonstrated in rat cells, whereas these activities could not be shown in fat cells from human omental and subcutaneous tissue. The findings for human cells are attributed to changes in cellular activity during preparation.
分离出的脂肪细胞用于研究胰岛素对人和大鼠脂肪组织中葡萄糖代谢的体外作用。在人皮下脂肪细胞中，当培养基中葡萄糖浓度为1至10微摩尔/毫升时，就能检测到胰岛素的作用。细胞反应性与葡萄糖水平呈反比。在每毫升葡萄糖浓度恒定为6微摩尔的情况下，研究了高达500微单位/毫升的不同浓度胰岛素的作用。在产生最大反应的最高浓度下，葡萄糖-U-(14)C向甘油酯甘油的转化率增加了100%，葡萄糖氧化增加了40%。剂量反应曲线在2至20微单位/毫升之间最陡。大鼠附睾脂肪细胞对胰岛素的反应性更强。在大鼠细胞中也能证明葡萄糖脂肪生成和戊糖循环活性，而在人网膜和皮下组织的脂肪细胞中则未显示出这些活性。人细胞的这些发现归因于制备过程中细胞活性的变化。
