Borgström B
J Lipid Res. 1967 Nov;8(6):598-608.
The composition of the emulsified oil and of the micellar phases obtained when a glyceride-fatty acid mixture is dispersed in bile salt solution has been defined. The micellar phase in equilibrium with the emulsified oil phase was obtained by filtration through Millipore filters. The behavior of different lipids in such systems was defined as the partition ratio, micellar/emulsified oil phase (m/o). Partition of fatty acids was found to be strongly dependent on the chain length of the fatty acid and the pH of the dispersion. The curve for partition against pH for oleic acid was interpreted to show a pK(a) for oleic acid in bile salt solution of approximately 7. The partition between micellar and oil phases is given for a series of lipids of different polarity. No significant difference in behavior was found for cholesterol and sitosterol. A relationship was found between the partition m/o and filtration rates through a Millipore filter in micellar solution. The lower the partition coefficient the lower was the rate of filtration. The results obtained are discussed in relation to the mechanism of absorption of fat from the small intestine.
当甘油酯 - 脂肪酸混合物分散在胆盐溶液中时所得到的乳化油和胶束相的组成已被确定。与乳化油相处于平衡状态的胶束相是通过微孔滤膜过滤得到的。不同脂质在这类体系中的行为被定义为分配比，即胶束相/乳化油相（m/o）。发现脂肪酸的分配强烈依赖于脂肪酸的链长和分散体系的pH值。油酸的分配与pH值关系曲线被解释为表明油酸在胆盐溶液中的pK(a)约为7。给出了一系列不同极性脂质在胶束相和油相之间的分配情况。胆固醇和谷甾醇在行为上没有发现显著差异。在胶束溶液中发现分配比m/o与通过微孔滤膜的过滤速率之间存在一种关系。分配系数越低，过滤速率越低。结合小肠脂肪吸收机制对所获得的结果进行了讨论。
