Feldman E B, Borgström B
Department of Physiological Chemistry, University of Lund, Lund, Sweden.
Lipids. 1966 Nov;1(6):430-8. doi: 10.1007/BF02532548.
Bile salt lipid emulsions were prepared which simulated the emulsified oil-micellar phase system of the small intestinal content during fat digestion.Application of such emulsions to gel columns prepared and eluted with 6 mM sodium taurodeoxycholate separated an emulsion phase and a micellar phase. The distribution of lipid solutes into the two phases under these conditions was measured.Micellar dimensions were larger as lipid concentrations were increased. Inclusion of multiple lipid classes resulted in larger micellar particles.Monoglyceride and fatty acids were eluted completely in the micellar phase under these conditions. Minimal measurable amounts of triolein were recovered in micellar solution. This was confirmed by extraction, chromatographic separation and quantitative analysis. As diolein concentration was increased, less was recovered in the micellar phase. When monoglyceride was added, more diolein entered the micellar phase. Addition of triglyceride enhanced the distribution of diolein into the emulsion phase.
制备了胆盐脂质乳剂，其模拟了脂肪消化过程中小肠内容物的乳化油 - 胶束相系统。将此类乳剂应用于用6 mM牛磺脱氧胆酸钠制备并洗脱的凝胶柱，分离出一个乳剂相和一个胶束相。测定了在此条件下脂质溶质在两相中的分布。随着脂质浓度的增加，胶束尺寸增大。包含多种脂质类别会导致形成更大的胶束颗粒。在这些条件下，甘油单酯和脂肪酸完全在胶束相中洗脱。在胶束溶液中回收的三油酸甘油酯量极少且可测量。这通过萃取、色谱分离和定量分析得到证实。随着二油精浓度的增加，在胶束相中回收的量减少。当加入甘油单酯时，更多的二油精进入胶束相。加入甘油三酯会增强二油精在乳剂相中的分布。
