Forget B G, Weissman S M
Science. 1967 Dec 29;158(3809):1695-9. doi: 10.1126/science.158.3809.1695.
The nucleotide sequence of 5S RNA derived from KB carcinoma cell ribosomes has been determined. The molecule has a length of either 120 or 121 nucleotides with uridine at its 3'-terminus and guanylic acid at its 5'-terminus. If, in addition to Watson-Crick base-pairing, one accepts occasional base-pairing of guanylic acid to uridylic acid, long sequences of complementary nucleotides can be identified within the molecule. Two regions of the molecule contain sequences complementary to four or five bases in the pentanucleotide sequence guanylic acid, ribothymidylic acid, pseudouridylic acid, cytidylic acid, guanylic acid, which is common to most transfer RNA molecules. This is the first time the sequence of an animal-cell RNA has been determined.
已确定源自KB癌细胞核糖体的5S RNA的核苷酸序列。该分子长度为120或121个核苷酸，3'-末端为尿苷，5'-末端为鸟苷酸。如果除了沃森-克里克碱基配对外，还接受鸟苷酸与尿苷酸的偶尔碱基配对，那么在分子内可以识别出长的互补核苷酸序列。该分子的两个区域包含与大多数转移RNA分子共有的五核苷酸序列鸟苷酸、核糖胸苷酸、假尿苷酸、胞苷酸、鸟苷酸中的四个或五个碱基互补的序列。这是首次确定动物细胞RNA的序列。
