Tobey R A, Anderson E C, Petersen D F
J Cell Biol. 1967 Oct;35(1):53-9. doi: 10.1083/jcb.35.1.53.
The generation time of a Chinese hamster cell line was varied by the use of different lots of sera in the culture media. Analysis of the division waves following thymidine synchronization showed that lengthening of the generation time was a result of an increase in duration of the G(1) phase and that thymidine treatment reduced the duration of G(1) back to its minimum value.
通过在培养基中使用不同批次的血清,改变了中国仓鼠细胞系的代时。对胸苷同步化后的分裂波进行分析表明,代时延长是G1期持续时间增加的结果,并且胸苷处理可将G1期的持续时间恢复到其最小值。