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Synthesis of RNA and protein during mitosis in mammalian tissue culture cells.哺乳动物组织培养细胞有丝分裂期间RNA和蛋白质的合成
Exp Cell Res. 1962 Mar;26:260-8. doi: 10.1016/0014-4827(62)90176-3.
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Normal distribution of cell generation rate.细胞生成率的正态分布。
Exp Cell Res. 1962 Mar;26:439-50. doi: 10.1016/0014-4827(62)90150-7.
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STUDIES OF THE LIFE CYCLE OF MAMMALIAN CELLS.哺乳动物细胞生命周期的研究。
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SYNCHRONIZED MAMMALIAN CELLS: AN EXPERIMENTAL TEST OF A MODEL FOR SYNCHRONY DECAY.同步化哺乳动物细胞：同步化衰退模型的实验测试
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QUANTITY PRODUCTION OF SYNCHRONIZED MAMMALIAN CELLS IN SUSPENSION CULTURE.悬浮培养中同步化哺乳动物细胞的批量生产。
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THE DECAY OF SYNCHRONIZATION OF CELL DIVISION.细胞分裂同步性的衰退
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LIFE CYCLE ANALYSIS OF MAMMALIAN CELLS. II. CELLS FROM THE CHINESE HAMSTER OVARY GROWN IN SUSPENSION CULTURE.哺乳动物细胞的生命周期分析。II. 悬浮培养的中国仓鼠卵巢细胞
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SYNTHESIS OF RNA AND PROTEIN REQUIRED FOR THE MITOSIS OF MAMMALIAN CELLS.哺乳动物细胞有丝分裂所需的RNA和蛋白质的合成。
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INHIBITION OF PROTEIN SYNTHESIS BY PUROMYCIN.嘌呤霉素对蛋白质合成的抑制作用。
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PROTEIN SYNTHESIS AND RNA SYNTHESIS DURING MITOSIS IN ANIMAL CELLS.动物细胞有丝分裂过程中的蛋白质合成与RNA合成。
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哺乳动物细胞的生命周期分析。3. 嘌呤霉素和放线菌素对中国仓鼠细胞分裂的抑制作用。

Life cycle analysis of mammalian cells. 3. The inhibition of division in Chinese hamster cells by puromycin and actinomycin.

作者信息

Tobey R A, Petersen D F, Anderson E C, Puck T T

出版信息

Biophys J. 1966 Sep;6(5):567-81. doi: 10.1016/s0006-3495(66)86678-x.

DOI:10.1016/s0006-3495(66)86678-x
PMID:5970563
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1368015/
Abstract

Analysis of the effects of actinomycin and puromycin on the G(2) and mitotic parts of the life cycle in Chinese hamster ovary cells grown in suspension and synchronized by thymidine treatment has been carried out. Rates of division of partially synchronized cell populations were measured in the presence and absence of the drugs, and various controls were performed to test for absence of complex side effects. Actinomycin produces a block 1.9 hr before completion of division, while puromycin produces a block almost coinciding with the initiation of mitosis. Evidence is presented that the puromycin block may be a double one, inhibiting one kind of protein synthesis that virtually coincides with the beginning of mitosis and another that occurs about 8 min earlier. The data are interpreted in terms of the time interval between messenger formation and its associated protein synthesis in this region of the life cycle. The various events studied have been provisionally mapped in the G(2) and mitotic periods of the life cycle.

摘要

已对放线菌素和嘌呤霉素对悬浮培养并用胸苷处理使其同步化的中国仓鼠卵巢细胞生命周期中G(2)期和有丝分裂期的影响进行了分析。在有和没有药物的情况下测量了部分同步化细胞群体的分裂速率，并进行了各种对照以测试是否不存在复杂的副作用。放线菌素在分裂完成前1.9小时产生阻滞，而嘌呤霉素产生的阻滞几乎与有丝分裂开始同时发生。有证据表明嘌呤霉素阻滞可能是双重阻滞，抑制一种几乎与有丝分裂开始同时发生的蛋白质合成以及另一种大约提前8分钟发生的蛋白质合成。根据生命周期这个区域中信使形成与其相关蛋白质合成之间的时间间隔对数据进行了解释。所研究的各种事件已暂时定位在生命周期的G(2)期和有丝分裂期。